Detection of blaKPC and blaGES Carbapenemase Genes in Klebsiella pneumoniae Isolated from Hospitalized Patients in Kashan, Iran.

Q3 Medicine
Farzaneh Firoozeh, Mehdi Aghaseyed-Hosseini, Mohammad Zibaei, Ahmad Piroozmand
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引用次数: 8

Abstract

Introduction: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are among the highly antimicrobial resistant gram negative bacteria and infections due to them are an increasingly major health problem worldwide.

Methods: In this study we have detected the blaKPC and blaGES carbapenemase genes in Klebsiella pneumoniae isolated from hospitalized patients in Kashan, Iran. In a cross-sectional study, a total of 181 K. pneumoniae isolates were recovered from clinical specimens during November 2013 to October 2014.

Result: Antimicrobial susceptibility profiles were determined using disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and CLSI guidelines. Carbapenem-resistant K. pneumoniae isolates were identified. PCR method and sequencing were used for detection of blaKPC and blaGES carbapenemase genes. Of the 181 K. pneumoniae isolates, 35 (19.3%) were found to be resistant to imipenem and 150 (82.9%) were identified as MDR strains. Among carbapenems, the most resistant rate 39 (21.5%) was seen against ertapenem using disk diffusion method. Of K. pneumoniae isolates 21 (11.6%) and 42 (23.2%) carried blaKPC and blaGES genes, respectively and 19(10.5%) carried both genes simultaneously.

Conclusion: The data of current study revealed that the frequency of resistance to carbapenems and production of carbapenemase enzymes especially GES type was high among clinical isolates of K pneumoniae in Kashan, Iran.

伊朗卡尚地区住院患者肺炎克雷伯菌blaKPC和blaGES碳青霉烯酶基因的检测
产碳青霉烯酶(KPC)的肺炎克雷伯菌是高度耐药的革兰氏阴性菌之一,由它们引起的感染是世界范围内日益严重的健康问题。方法:对伊朗卡尚地区住院患者分离的肺炎克雷伯菌进行blaKPC和blaGES碳青霉烯酶基因检测。在横断面研究中,2013年11月至2014年10月从临床标本中共分离出181株肺炎克雷伯菌。结果:按照欧洲药物敏感性试验委员会(EUCAST)和CLSI指南,采用纸片扩散法测定药物敏感性。鉴定出耐碳青霉烯肺炎克雷伯菌分离株。采用PCR法和测序法检测blaKPC和blaGES碳青霉烯酶基因。181株肺炎克雷伯菌中,亚胺培南耐药35株(19.3%),耐多药150株(82.9%)。纸片扩散法对厄他培南的耐药率最高,为21.5%。肺炎克雷伯菌分离株中分别携带blaKPC和blaGES基因21株(11.6%)和42株(23.2%),同时携带blaKPC和blaGES基因19株(10.5%)。结论:目前的研究资料显示,伊朗卡尚地区临床分离的肺炎克雷伯菌对碳青霉烯类药物的耐药频率和碳青霉烯类酶的产生频率较高,尤其是GES型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Recent patents on anti-infective drug discovery
Recent patents on anti-infective drug discovery Medicine-Pharmacology (medical)
CiteScore
2.40
自引率
0.00%
发文量
1
期刊介绍: Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.
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