Correlation Between qacE and qacE∆1 Efflux Pump Genes, Antibiotic and Disinfectant Resistant Among Clinical Isolates of E.coli.

Q3 Medicine

Recent patents on anti-infective drug discovery Pub Date : 2016-01-01 DOI:10.2174/1574891X11666160815094718

Maryam Shafaati, Mohammadali Boroumand, Jamileh Nowroozi, Pouya Amiri, Hossein Kazemian

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引用次数: 22

Abstract

Introduction: Antiseptics and disinfectants have been used widely in hospitals and other health care settings to control the growth of microorganisms. However, some disinfectant resistant strains were reported. The objectives of our study were to evaluate correlation between the efflux pump genes, drugs and disinfectant resistant among clinical isolates of E.coli.

Methods: A total of 102 of E. coli strains were isolated from urine sample of hospitalized patients. The antibiotic susceptibility was carried out by disc diffusion method. Didecyl di-methyl ammonium chloride (DDDMAC) was used as Quaternary ammonium compound (QAC) disinfectant which was used in Heart Center Hospital. PCR reaction was carried out for detection of qacE and qac∆E efflux pump genes.

Result: Almost all the strains had higher resistance to ampicillin, ciproflaxacin, cotrimaxazole and cephalothin. Totally 49% (n: 50) of strains were produced ESBL. Almost all the strains have MIC value between 0.00195 to 0.0078 mg/l for DDDMAC. Correlation between presence of qacE and qac∆E genes and antibiotic resistance was perceived. Presence of qacE and qac∆E genes among strains that have high disinfectant MIC value were 96.9% and 93.7% respectively. In addition, 98% of ESBL producing strains harbored qacE gene and 94% of ESBL producing strains harbored qac∆E gene.

Conclusion: Our study indicated that there was a strong correlation between presence of qacE and qac∆E genes with resistance to some antibiotics and growth in media which contain high concentration of disinfectant. In conclusion, other mechanisms also play important role in resistant to antimicrobial agents but the role of efflux pumps in resistant to antimicrobial agents should not be neglected.

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临床分离大肠杆菌qacE、qacE∆1外排泵基因与抗生素、消毒剂耐药性的相关性

导言:防腐剂和消毒剂已广泛用于医院和其他卫生保健机构，以控制微生物的生长。然而，也有一些耐消毒剂菌株的报道。目的:探讨大肠杆菌临床分离株外排泵基因与药物及消毒剂耐药性的相关性。方法:从住院患者尿液中分离出102株大肠杆菌。采用纸片扩散法进行药敏试验。采用二癸基二甲基氯化铵(DDDMAC)作为季铵盐消毒剂在心脏中心医院应用。采用PCR反应检测qacE和qac∆E外排泵基因。结果:几乎所有菌株对氨苄西林、环丙沙星、复方新美唑和头孢菌素均有较高的耐药性。共有49%(50株)的菌株产生了ESBL。几乎所有菌株对DDDMAC的MIC值都在0.00195 ~ 0.0078 mg/l之间。qacE和qac∆E基因的存在与抗生素耐药性存在相关性。消毒MIC值较高的菌株中qacE和qac∆E基因的检出率分别为96.9%和93.7%。此外，98%的ESBL产菌携带qacE基因，94%的ESBL产菌携带qac∆E基因。结论:我们的研究表明，qacE和qac∆E基因的存在与某些抗生素的耐药性和在含有高浓度消毒剂的培养基中的生长有很强的相关性。综上所述，其他机制在抗微生物药物耐药中也起重要作用，但外排泵在抗微生物药物耐药中的作用不容忽视。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Recent patents on anti-infective drug discovery Medicine-Pharmacology (medical)

CiteScore

2.40

自引率

0.00%

发文量

期刊介绍： Recent Patents on Anti-Infective Drug Discovery publishes review articles on recent patents in the field of anti-infective drug discovery e.g. novel bioactive compounds, analogs & targets. A selection of important and recent patents on anti-infective drug discovery is also included in the journal. The journal is essential reading for all researchers involved in anti-infective drug design and discovery.