{"title":"Impairment and Regeneration of Gastric Mucosa After Irradiation in Mice.","authors":"Huihong Zeng, Jinying Tong, Jianghong Zou, Aixiang Fu, Li-Jian Shao, Qingxian Zhu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To understand the effects of γ-ray irradiation (IR) on the proliferation of gastric mucosal cells and to investigate the possible mechanisms that affect gastric mucosal cell proliferation.</p><p><strong>Study design: </strong>C57BL/6J mice were exposed to IR at various doses (4, 8, and 15 Gy). We measured the changes of gastric mucosal BrdU-positive cells and the expression of β-catenin protein in the isthmus of fundic glands at days 1, 3, and 5 after irradiation.</p><p><strong>Results: </strong>Our data showed that the mice that received 15 Gy IR died within 4 days. IR caused gastric mucosal injury in mice, and the degree of injury increased along with the increasing doses. Compared with the control group, the proliferation of gastric mucosal cells was inhibited 1 day after irradiation. Cell proliferation was recovered on day 5 after low-dose (4 and 8 Gy) IR, while proliferation was continuously inhibited after high-dose (15 Gy) IR. β-catenin expression was increased and had a translocation in the isthmus of gastric mucosa.</p><p><strong>Conclusion: </strong>These findings suggest that gastric mucosa is sensitive to irradiation. The Wnt/β-catenin pathway is activated and plays a role in cell proliferation of gastric mucosa upon irradiation.</p>","PeriodicalId":55517,"journal":{"name":"Analytical and Quantitative Cytopathology and Histopathology","volume":"37 3","pages":"169-76"},"PeriodicalIF":0.1000,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Quantitative Cytopathology and Histopathology","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
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Abstract
Objective: To understand the effects of γ-ray irradiation (IR) on the proliferation of gastric mucosal cells and to investigate the possible mechanisms that affect gastric mucosal cell proliferation.
Study design: C57BL/6J mice were exposed to IR at various doses (4, 8, and 15 Gy). We measured the changes of gastric mucosal BrdU-positive cells and the expression of β-catenin protein in the isthmus of fundic glands at days 1, 3, and 5 after irradiation.
Results: Our data showed that the mice that received 15 Gy IR died within 4 days. IR caused gastric mucosal injury in mice, and the degree of injury increased along with the increasing doses. Compared with the control group, the proliferation of gastric mucosal cells was inhibited 1 day after irradiation. Cell proliferation was recovered on day 5 after low-dose (4 and 8 Gy) IR, while proliferation was continuously inhibited after high-dose (15 Gy) IR. β-catenin expression was increased and had a translocation in the isthmus of gastric mucosa.
Conclusion: These findings suggest that gastric mucosa is sensitive to irradiation. The Wnt/β-catenin pathway is activated and plays a role in cell proliferation of gastric mucosa upon irradiation.
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