Conjunctival TGF-B Level in Primary Augmented Trabeculectomy.

IF 0.4 Q4 OPHTHALMOLOGY

Open Ophthalmology Journal Pub Date : 2015-07-31 eCollection Date: 2015-01-01 DOI:10.2174/1874364101509010136

Guan Fook Ng, Ishak Siti Raihan, Yaakub Azhany, Che Hussin Che Maraina, K Gurusamy Banumathi, Tajudin Liza-Sharmini

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引用次数: 6

Abstract

Purpose: To compare the levels of conjunctival transforming growth factor beta (TGF-β) between glaucoma and control patients and to determine conjunctival TGF-β levels before and 3 months after augmented primary trabeculectomy.

Methods: Patients with primary open angle glaucoma (POAG) or primary angle closure glaucoma (PACG) admitted for mitomycin (MMC) augmented primary trabeculectomy due to failure in achieving target pressure after maximum medical therapy were selected. Age-matched non-glaucoma patients were controls. Impression cytology of the conjunctiva was obtained twice from glaucoma patients (1 week before augmented primary trabeculectomy and 3 months after surgery) and once from controls. Conjunctival cells were tagged with an anti-TGF-β antibody and analyzed by flow cytometry.

Results: Eighteen patients (11 POAG and 7 PACG patients) and 18 age-matched control patients were included. Conjunctival TGF-β levels were significantly different between glaucoma (35.21% ± 14.12%) and control patients (14.96% ± 6.34%) (p = 0.001). There was a significant reduction in conjunctival TGF-β levels after augmented trabeculectomy (23.0% ± 13.8%) (p < 0.001). A significantly greater reduction in conjunctival TGF-β levels (61.6% ± 17.9%) was associated with complete success of trabeculectomy at 3 months (83.3%) after surgical intervention (p = 0.029).

Conclusion: The reduction of TGF-β on the conjunctival post primary augmented trabeculectomy may suggest TGF-β as potential predicting marker of short term trabeculectomy success. However, the result may be affected by site of impression, topical pressure lowering drugs and small sample size.

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原发性增强小梁切除术中结膜TGF-B水平的变化。

目的:比较青光眼患者与对照组结膜转化生长因子β (TGF-β)水平，测定原发性增强小梁切除术前及术后3个月结膜TGF-β水平。方法:选择原发性开角型青光眼(POAG)或原发性闭角型青光眼(PACG)患者行丝裂霉素(MMC)增强原发性小梁切除术，经最大药物治疗后未能达到目标压力。对照组为年龄匹配的非青光眼患者。对青光眼患者进行两次结膜印迹细胞学检查(增强原发性小梁切除术前1周和术后3个月)，对对照组进行一次。结膜细胞用抗tgf -β抗体标记，流式细胞术分析。结果:纳入18例患者(POAG 11例，PACG 7例)和18例年龄匹配的对照患者。青光眼患者结膜TGF-β水平(35.21%±14.12%)与对照组(14.96%±6.34%)差异有统计学意义(p = 0.001)。增强小梁切除术后结膜TGF-β水平显著降低(23.0%±13.8%)(p < 0.001)。结膜TGF-β水平显著降低(61.6%±17.9%)与手术干预后3个月小梁切除术完全成功(83.3%)相关(p = 0.029)。结论:原发性增强小梁切除术后结膜TGF-β水平的降低提示TGF-β可作为短期小梁切除术成功与否的潜在预测指标。然而，结果可能受到印模部位，局部降压药物和小样本量的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Ophthalmology Journal OPHTHALMOLOGY-

CiteScore

0.50

自引率

0.00%

发文量

期刊介绍： The Open Ophthalmology Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, letters and guest edited single topic issues in all important areas of experimental and clinical research in ophthalmology, including use of ophthalmological therapies, devices and surgical techniques. The Open Ophthalmology Journal, a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and making them freely available to researchers worldwide.