Emerging Applications for Optically Enabled Intravital Microscopic Imaging in Radiobiology.

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Molecular Imaging Pub Date : 2015-01-01
Azusa Maeda, Iris Kulbatski, Ralph S DaCosta
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引用次数: 0

Abstract

Radiation therapy is an effective cancer treatment used in over 50% of cancer patients. Preclinical research in radiobiology plays a major role in influencing the translation of radiotherapy-based treatment strategies into clinical practice. Studies have demonstrated that various components of tumors and their microenvironments, including vasculature, immune and stem cells, and stromal cells, can influence the response of solid tumors to radiation. Optically enabled imaging techniques used in experimental animal models of cancer offer a unique and powerful way to quantitatively track spatiotemporal changes in these tumor components in vivo at macro-, meso-, and microscopic resolutions following radiotherapy. In this review, we discuss the role of both well-established and emerging intravital microscopy techniques for studying tumors and their microenvironment in vivo, in response to irradiation. The development and application of new animal models, small animal microirradiation technologies, and multimodal optically enabled intravital microscopy techniques are emphasized within the framework of preclinical radiobiology research. We also comment on the potential influence that these newer imaging techniques may have on the clinical translation of new preclinical radiobiology discoveries.

光学活体显微成像在放射生物学中的新应用。
放射治疗是一种有效的癌症治疗方法,用于50%以上的癌症患者。放射生物学的临床前研究在影响以放射治疗为基础的治疗策略转化为临床实践方面起着重要作用。研究表明,肿瘤及其微环境的各种组成部分,包括脉管系统、免疫细胞和干细胞以及基质细胞,可影响实体肿瘤对辐射的反应。在实验动物癌症模型中使用的光学成像技术提供了一种独特而强大的方法来定量跟踪这些肿瘤成分在放射治疗后的宏、中观和微观分辨率的时空变化。在这篇综述中,我们讨论了成熟的和新兴的活体显微技术在研究肿瘤及其体内微环境对辐照的反应中的作用。在临床前放射生物学研究的框架内,强调了新动物模型,小动物微辐照技术和多模态光学活体显微镜技术的开发和应用。我们还评论了这些较新的成像技术可能对临床前放射生物学新发现的临床转化产生的潜在影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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