β-ESTRADIOL INDUCES CYTOTOXIC EFFECTS TO HUMAN T-LYMPHOMA (JURKAT) CELLS THROUGH OXIDATIVE STRESS.

Abstract

β-estradiol is the most potent estrogen of a group of endogenous estrogen steroids which includes estrone and estriol. This steroid hormone is the most potent natural estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Although β-estradiol protects the renal and cardiovascular systems, the mechanisms involved remain unclear. In this research, we performed the MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay to evaluate the effect of β-estradiol on human T-lymphoma (Jurkat) cells upon 24 and 48 hours, respectively. Lipid peroxidation assay was also performed to estimate the levels of malondialdehyde (MDA) production in β-estradiol-treated cells. The results of MTT assay demonstrated that low, physiological levels of β-estradiol induce cellular proliferation in Jurkat T-cells. At higher dose of exposure, β-estradiol decreases the viability of Jurkat T-cells compared to the control cells. Data generated from lipid peroxidation assay resulted in a significant increase (p < 0.05) in MDA production in β-estradiol treated sample. Upon 48 h of exposure, MDA concentrations in the sample [µM] (mean ±SE, n = 3) compared to untreated control were 4.9 ± 1.7, 8.1 ± 1,6 11.5 ± 2.2, 21.1 ± 2.3, 19.5 ± 1.4, and 21.5 ± 2.6 in 0, 1, 2, 4, 8, and 16 µM β-estradiol, respectively. In summary, findings from this study demonstrated that high dose of β-estradiol is cytotoxic to Jurkat T-cells. This cytotoxicity is found to be associated with oxidative stress.