Colonization and Persistence of Labeled and "Foreign" Strains of Aggregatibacter actinomycetemcomitans Inoculated into the Mouths of Rhesus Monkeys.

Daniel H Fine, Maribasappa Karched, David Furgang, Vandana Sampathkumar, Senthil Velusamy, Dipti Godboley
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引用次数: 11

Abstract

Aggregatibacter actinomycetemcomitans (Aa) is a pathobiont and part of a consortium of bacteria that can lead to periodontitis in humans. Our aim was to develop a model for oral inoculation of labeled Aa into a suitable host in order to study Aa traits and ecological factors that either enhance or repress its persistence. Primate species were screened for Aa to select a host for colonization studies. Macaca mulatta (Rhesus/Rh) was selected. Rh Aa strains were isolated, subjected to sequencing and functional analysis for comparison to human strains. "Best" methods for microbial decontamination prior to inoculation were assessed. Three groups were studied; Group 1 (N=5) was inoculated with Aa Spectinomycin resistant (SpecR) Rh strain 4.35, Group 2 (N=5) inoculated with Aa SpecR human strain IDH 781, and Group 3 (N=5) the un-inoculated control. Repeated feeding with pancakes spiked with SpecRAa followed high dose oral inoculation. Cheek, tongue, and plaque samples collected at baseline 1, 2, 3, and 4 weeks after inoculation were plated on agar; 1) selective for Aa, 2) enriched for total counts, and 3) containing 50 µg/ml of Spec. Aa was identified by colonial morphology and DNA analysis. Rh and human Aa had > 93-98 % genome identity. Rh Aa attached to tissues better than IDH 781 in vitro (p < 0.05). SpecR IDH 781 was not recovered from any tissue at any time; whereas, RhSpecR 4.35 was detected in plaque, but never tongue or cheek, in all monkeys at all times (> 1 × 105 colonies/ml; p < 0.001). In conclusion, the primate model provides a useful platform for studying integration of Aa strains into a reduced but established oral habitat. Primate derived SpecRAa was consistently detected in plaque at all collection periods; however, human derived Aa was never detected. The model demonstrated both microbial as well as tissue specificity.

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标记和“外来”放线菌聚集菌在恒河猴口腔内的定植和持久性。
放线菌群(放线菌群)是一种致病菌,是可导致人类牙周炎的细菌联合体的一部分。我们的目的是建立一个经口接种标记Aa的模型,以研究Aa的性状和生态因子对其持久性的影响。对灵长类动物进行Aa筛选,选择寄主进行定殖研究。选择猕猴(恒河猴/Rh)。分离Rh Aa菌株,进行测序和功能分析,与人类菌株进行比较。评估了接种前微生物去污的“最佳”方法。研究了三组;1组(N=5)接种Aa SpecR耐药(SpecR) Rh菌株4.35,2组(N=5)接种Aa SpecR人株IDH 781, 3组(N=5)为未接种的对照。在高剂量口服接种后,用加有SpecRAa的煎饼反复喂养。接种后1、2、3和4周收集的脸颊、舌头和菌斑样本涂在琼脂上;1)对Aa有选择性,2)总计数丰富,3)含有50µg/ml的Spec. Aa,通过菌落形态和DNA分析鉴定。Rh与人类Aa具有> 93- 98%的基因组同源性。Rh Aa体外对组织的附着性优于IDH 781 (p < 0.05)。在任何时候都没有从任何组织中提取到SpecR IDH 781;而在所有猴子的牙菌斑中均检测到RhSpecR 4.35,但在舌头和脸颊中均未检测到(> 1 × 105菌落/ml);P < 0.001)。总之,灵长类动物模型为研究Aa菌株在减少但已建立的口腔栖息地中的整合提供了一个有用的平台。灵长类动物衍生的SpecRAa在所有收集期的斑块中都被一致检测到;但未检测到人源性Aa。该模型显示了微生物和组织的特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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