[APPLICATION OF FLOW CYTOMETRY FOR THE ANALYSIS OF CIRCULATING HEMOCYTE POPULATIONS IN THE ASCIDIAN HALOCYNTHIA AURANTIUM (PALLAS, 1787)].

A N Sukhachev, I S Dyachkov, I V Kudryavtsev, V V Kumeiko, A V Tsybulskiy, A V Polevshchikov
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Abstract

This study addresses the potentialities of flow cytometry in analyzing the composition of circulating hemocyte populations in the ascidian Halocynthia aurantium (Pallas, 1787) both using monoclonal antibodies (mAbs) against some human leukocyte conservative adhesion molecules and without mAbs. Flow cytometry, based on the assessment of forward and side scattering revealed five hemocyte populations. From the wide panel of antibodies against human leukocyte adhesion molecules (CD15, CD29, CD34, CD54, CD62L, CD62P, CD90, CD94, CD117, CD 166), only two mAbs (against CD54, CD90) displayed cross-reactivity with the H. aurantium hemocyte surface antigens. Distribution patterns of these antigens across the hemocyte populations have been analyzed.

[应用流式细胞术分析海鞘(ascidian halocynthia aurantium)的循环血细胞群[pallas, 1787]。
本研究探讨了流式细胞术在分析海鞘(Halocynthia aurantium, Pallas, 1787)循环血细胞群组成方面的潜力,使用单克隆抗体(mab)对抗一些人类白细胞保守粘附分子和不使用单克隆抗体。流式细胞术,基于前向和侧向散射的评估显示了五个血细胞群。从广泛的抗人白细胞粘附分子抗体(CD15、CD29、CD34、CD54、CD62L、CD62P、CD90、CD94、CD117、cd166)中,只有两种单克隆抗体(抗CD54、CD90)与H. aurantium血细胞表面抗原显示交叉反应性。这些抗原在血细胞群中的分布模式已被分析。
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