Syndecan-4 is More Sensitive in Detecting Hypertensive Left Ventricular Diastolic Dysfunction in 2K2C Rats.

IF 1.9 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

International Journal of Hypertension Pub Date : 2022-09-24 eCollection Date: 2022-01-01 DOI:10.1155/2022/1447425

Wenyue Dai, Yanqiu Liu, Fengjuan Yao, Wei Li, Jia Liu, Cuiling Li, Donghong Liu

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Abstract

Objective: The aim of this study was to investigate the changes of syndecan-4 (SDC-4) during the hypertensive period in two kidney-two clip (2K2C) hypertension rats and compare them to brain natriuretic peptide (BNP) and the echocardiographic parameters for diastolic function evaluation in the rat model of 2K2C hypertension.

Methods: A total of 36 Sprague-Dawley (SD) rats were used in this study. Hypertension was induced in 21 by 2K2C surgery, and 15 were sham-operated. Both the 2K2C hypertension group (n = 21) and the sham-operated group (n = 15) were equally divided into 3 subgroups according to the schedules (week 4, week 8, and week 12). Serum SDC-4 and BNP were detected by ELISA, and echocardiography indexes were acquired.

Results: The level of SDC-4 and cardiac fibrosis increased gradually as the experiment was processed, and BNP, Tei index, and E/E' followed to be raised as high blood pressure was maintained after four weeks in the 2K2C hypertension rats. In the earlier 4 weeks, only SDC-4 and cardiac fibrosis were significantly increased in 2K2C hypertensive rats in comparison with normotensive rats. And it was shown that SDC-4 was positively correlated with BNP level during the entire study (r = 0.762, p < 0.01).

Conclusion: SDC-4 increases gradually during the process of diastolic dysfunction in 2K2C hypertensive rats. SDC-4 is the earliest biomarker reflecting diastolic dysfunction in this model, superior to E/E' and the Tei index. Our results indicate that serum SDC-4 could act as an early biomarker to show diastolic dysfunction.

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Syndecan-4 对检测 2K2C 大鼠高血压左心室舒张功能障碍更敏感

研究目的本研究旨在探讨双肾-双夹（2K2C）高血压大鼠在高血压期间辛迪加-4（SDC-4）的变化，并将其与脑钠肽(BNP)和超声心动图参数进行比较，以评估2K2C高血压大鼠模型的舒张功能：本研究共使用了 36 只 Sprague-Dawley (SD) 大鼠。方法：本研究共使用了 36 只 Sprague-Dawley (SD) 大鼠，其中 21 只通过 2K2C 手术诱发了高血压，15 只进行了假手术。2K2C 高血压组（n = 21）和假手术组（n = 15）按计划（第 4 周、第 8 周和第 12 周）平均分为 3 个亚组。用ELISA法检测血清SDC-4和BNP，并采集超声心动图指标：结果：随着实验的进行，SDC-4和心脏纤维化的水平逐渐升高，2K2C高血压大鼠的BNP、Tei指数和E/E'在高血压维持四周后随之升高。与正常血压大鼠相比，2K2C 高血压大鼠在前 4 周只有 SDC-4 和心脏纤维化显著增加。在整个研究过程中，SDC-4 与 BNP 水平呈正相关（r = 0.762，p < 0.01）：结论：SDC-4 在 2K2C 高血压大鼠舒张功能障碍过程中逐渐增加。结论：SDC-4 在 2K2C 高血压大鼠舒张功能障碍过程中逐渐升高，是该模型中最早反映舒张功能障碍的生物标志物，优于 E/E' 和 Tei 指数。我们的研究结果表明，血清 SDC-4 可作为显示舒张功能障碍的早期生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Hypertension Medicine-Internal Medicine

CiteScore

4.00

自引率

5.30%

发文量

期刊介绍： International Journal of Hypertension is a peer-reviewed, Open Access journal that provides a forum for clinicians and basic scientists interested in blood pressure regulation and pathophysiology, as well as treatment and prevention of hypertension. The journal publishes original research articles, review articles, and clinical studies on the etiology and risk factors of hypertension, with a special focus on vascular biology, epidemiology, pediatric hypertension, and hypertensive nephropathy.