Debbie van der Burg, Hermann Wätzig, Cari E Sänger-van de Griend
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Abstract
This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100 mM phosphoric acid and 55 mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1 M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10-110 µM for undiluted samples, with 10 μM being the lower range limit of quantification QL, showed accuracy recoveries of 94-104%, and migration time and peak area repeatabilities within 0.4% RSD and 2.6% RSD, respectively.
期刊介绍：
Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical.
Subject areas include (but are by no means limited to):
Separation
Spectroscopy
Mass spectrometry
Chromatography
Analytical Sample Preparation
Electrochemical analysis
Hyphenated techniques
Data processing
As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
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