Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Omprakash Sarkar, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas
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引用次数: 3

Abstract

The present study reports the mixed culture acidogenic production of biohydrogen and carboxylic acids (CA) from brewery spent grains (BSG) in the presence of high concentrations of cobalt, iron, nickel, and zinc. The metals enhanced biohydrogen output by 2.39 times along with CA biosynthesis by 1.73 times. Cobalt and iron promoted the acetate and butyrate pathways, leading to the accumulation of 5.14 gCOD/L of acetic and 11.36 gCOD/L of butyric acid. The production of solvents (ethanol + butanol) was higher with zinc (4.68 gCOD/L) and cobalt (4.45 gCOD/L). A combination of all four metals further enhanced CA accumulation to 42.98 gCOD/L, thus surpassing the benefits accrued from supplementation with individual metals. Additionally, 0.36 and 0.31 mol green ammonium were obtained from protein-rich brewery spent grain upon supplementation with iron and cobalt, respectively. Metagenomic analysis revealed the high relative abundance of Firmicutes (>90%), of which 85.02% were Clostridium, in mixed metal-containing reactors. Finally, a significant correlation of dehydrogenase activity with CA and biohydrogen evolution was observed upon metal addition.

Abstract Image

金属对酿酒酒糟产酸代谢促进产氢和羧酸的影响:微生物动力学和生化分析
本研究报道了在高浓度钴、铁、镍和锌存在的情况下,从啤酒废谷物(BSG)中产生生物氢和羧酸(CA)的混合培养。金属使生物氢产量提高了2.39倍,CA生物合成提高了1.73倍。钴和铁促进了乙酸和丁酸途径,导致乙酸和丁酸积累分别为5.14 gCOD/L和11.36 gCOD/L。锌(4.68 gCOD/L)和钴(4.45 gCOD/L)的溶剂(乙醇+丁醇)产量较高。这四种金属的组合进一步提高了CA积累量,达到42.98 gCOD/L,从而超过了补充单个金属所带来的益处。添加铁和钴后,富蛋白啤酒废粮的绿铵含量分别为0.36 mol和0.31 mol。宏基因组分析显示,在混合含金属反应器中,厚壁菌门的相对丰度较高(>90%),其中梭状芽孢杆菌的丰度为85.02%。最后,在添加金属后,脱氢酶活性与CA和生物氢析出有显著的相关性。
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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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