Rotavirus Quantification in Children with Acute Gastroenteritis.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2023-01-01 Epub Date: 2022-10-14 DOI:10.1159/000526839

Ilaria Galliano, Valentina Daprà, Maddalena Dini, Martina Gavatorta, Allegra Sardo, Giada Lo Curcio, Massimiliano Bergallo

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引用次数: 0

Abstract

Introduction: Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aimed to quantify rotavirus (RV) with TaqMan real-time PCR in fecal samples of children with acute gastroenteritis (AGE) in accordance with the program of reduction of drug resistance and use of antibiotics.

Methods: A total of 190 fecal specimens were collected from under-5-year-old children with AGE in pediatric Hospital Regina Margherita of Turin in Italy in 2017. A total of 38 out of 67 (56.7%) episodes of AGE were associated with RV genomic detection with a multiplex commercial kit.

Results: All fecal specimens were tested for the presence of RV and other GE viruses. The most commonly detected virus was norovirus (41%), astrovirus (15.8%), human bocavirus (8.9%), sapovirus (7.9%), human parechovirus (5.8%), rhinovirus (4.2%), and adenovirus (1%). In 66 out of 190 (34.7%), RV was detectable with the median viral load 7.2E + 11 ± 60E + 11 genomes/mg fecal specimens.

Discussion/conclusions: Our results showed that RV was present in around 34.7% of children hospitalized for AGE, a rate similar to those reported in previous studies conducted elsewhere which were in the range of 33-75%. Our protocols are able to quantify the absolute number of viral particle/mg of feces. The clinical utility of quantitative molecular assays, such as RV viral load, will be markedly improved.

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急性肠胃炎患儿的轮状病毒定量。

导言：无论病原体是什么，大多数难治性腹泻都要接受抗生素治疗。本研究旨在根据减少抗药性和抗生素使用的计划，利用 TaqMan 实时 PCR 技术对急性肠胃炎（AGE）患儿粪便样本中的轮状病毒（RV）进行定量分析：2017年，意大利都灵Regina Margherita儿科医院共收集了190份5岁以下急性肠胃炎患儿的粪便标本。在67例AGE中，共有38例（56.7%）与使用多重商业试剂盒进行的RV基因组检测有关：对所有粪便标本进行了 RV 和其他 GE 病毒检测。最常检测到的病毒是诺如病毒（41%）、星状病毒（15.8%）、人博卡病毒（8.9%）、沙波病毒（7.9%）、人帕奇病毒（5.8%）、鼻病毒（4.2%）和腺病毒（1%）。190份粪便标本中有66份（34.7%）检测到RV，病毒载量中位数为7.2E + 11 ± 60E + 11基因组/毫克：我们的研究结果表明，因 AGE 住院的儿童中约有 34.7% 存在 RV，这一比例与之前在其他地方进行的研究中报告的 33%-75% 的比例相似。我们的方案能够量化每克粪便中病毒颗粒的绝对数量。定量分子检测（如 RV 病毒载量）的临床实用性将显著提高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567

期刊介绍： ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/Abstracted： Web of Science SCIE Scopus CAS INSPEC Portico