Peripheral Blood Expressions of MicroRNA-146a and MicroRNA-218 in Chronic Obstructive Pulmonary Disease with/without Cigarette Smoke Exposure.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Ebrahim Nadi, Ghazal Geramirad, Zohreh Kahramfar, Ashkan Rasuli-Saravani, Ghasem Solgi
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Abstract

Altered expression and dysregulation of microRNAs (miRNAs) have been reported in different samples of chronic obstructive pulmonary disease (COPD) patients. The present study attempted to evaluate the peripheral expressions of miR-146a and miR-218 in COPD patients and sex-matched healthy controls with/without cigarette smoke exposure (CSE). In this case-control study, blood samples were collected from 60 COPD patients (30 with CSE and 30 non-CSE in each group) and 60 healthy controls. Peripheral expressions of miRNA-146a and miR-218a were measured using qRT-PCR and results were compared between cases and controls as well as within the subgroups of patients. We found significantly decreased expressions for both miRNAs in the patients compared to healthy controls. Remarkable underexpression of miRNA-146a and miRNA-218 were found in the CSE and non-CSE patients compared to non-CSE healthy controls and even in the CSE versus non-CSE controls. Both groups of patients showed underexpression of two miRNAs in comparison with CSE healthy controls and interestingly, similar decrements were observed in the CSE versus non-CSE patients. Also, ROC curve analysis revealed the significantly diagnostic powers for both miRNAs in discrimination of patients from healthy individuals and CSE-COPD from non-CSE COPD patients. The underexpression of miR-146a and miR-218 in COPD patients and relation to CSE can be indicative of CSE-induced changes in miRNA expression profile and potential for these biomarkers in COPD risk assessment, particularly in those patients with CSE.

MicroRNA-146a和MicroRNA-218在慢性阻塞性肺疾病有/无香烟烟雾暴露中的外周血表达
在慢性阻塞性肺疾病(COPD)患者的不同样本中报道了microRNAs (miRNAs)的表达改变和失调。本研究试图评估有/没有香烟烟雾暴露(CSE)的COPD患者和性别匹配的健康对照中miR-146a和miR-218的外周表达。在这项病例对照研究中,收集了60名COPD患者(每组30名患有CSE, 30名非CSE)和60名健康对照者的血液样本。使用qRT-PCR检测miRNA-146a和miR-218a的外周表达,并比较病例与对照组之间以及患者亚组内的结果。我们发现,与健康对照组相比,患者中这两种mirna的表达显著降低。与非CSE健康对照相比,在CSE和非CSE患者中发现了显著的miRNA-146a和miRNA-218的低表达,甚至在CSE与非CSE对照中也是如此。与CSE健康对照组相比,两组患者均表现出两种mirna的低表达,有趣的是,在CSE患者与非CSE患者中观察到类似的减少。此外,ROC曲线分析显示,mirna在区分健康患者和CSE-COPD患者与非CSE-COPD患者方面具有显著的诊断能力。miR-146a和miR-218在COPD患者中的低表达及其与CSE的关系可以指示CSE诱导的miRNA表达谱的变化以及这些生物标志物在COPD风险评估中的潜力,特别是在那些患有CSE的患者中。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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