Identification of the genetic central dogma in osteogenic differentiation of MSCs by osteoinductive medium from transcriptional data sets

Q1 Medicine

Chronic Diseases and Translational Medicine Pub Date : 2022-05-31 DOI:10.1002/cdt3.26

Tong-Meng Jiang

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引用次数: 4

Abstract

Background

The genetic central dogma (GCD) has been demonstrated its essential function in many biological processes and diseases. However, its roles in the process of osteogenic differentiation of mesenchymal stem cells (MSCs) remain unclear.

Methods

In this project, we analyzed an online database of osteogenic differentiation of MSCs after 14 days and 28 days by osteoinductive medium (GSE83770). The differentially expressed genes were screened by GEO2R, with further conducting of KEGG pathways using DAVID. In addition, protein–protein interactions of the enriched pathways were performed using STRING with marked hub genes measured by the CytoHubba. Hub genes were verified by quantitative reverse-transcription polymerase chain reaction.

Results

Results showed that six pathways related to GCD, including DNA replication, Aminoacyl-tRNA biosynthesis, Mismatch repair, Ribosome, Spliceosome, and RNA degradation pathways enriched in the early stage (14 days vs. undifferentiated MSCs) of osteogenesis. The Lysosome pathway was highly enriched in the late stage (28 vs. 14 days) of osteogenesis, and Ribosome pathway plays a key role throughout the entire process (28 days vs. undifferentiated MSCs) of osteogenesis.

Conclusion

Both DNA replication and protein translation were functionally worked in the early stage of osteogenesis, whereas the Lysosome pathway was the only GCD-related one in the late stage of osteogenesis. The GCD-related Ribosome pathway occupied the entire process of osteogenesis.

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从转录数据集鉴定成骨诱导培养基中MSCs成骨分化的遗传中心规律

遗传中心规律(GCD)在许多生物过程和疾病中发挥着重要作用。然而，其在间充质干细胞成骨分化过程中的作用尚不清楚。方法在本项目中，我们分析了MSCs在骨诱导培养基(GSE83770) 14天和28天后成骨分化的在线数据库。通过GEO2R筛选差异表达基因，通过DAVID进一步传导KEGG通路。此外，利用STRING与CytoHubba测量的标记枢纽基因进行了富集途径的蛋白-蛋白相互作用。Hub基因通过定量逆转录聚合酶链反应验证。结果发现，在成骨早期(与未分化MSCs相比14天)，DNA复制、氨基酰基- trna生物合成、错配修复、核糖体、剪接体和RNA降解等6条与GCD相关的通路富集。溶酶体途径在成骨后期(28天和14天)高度富集，核糖体途径在整个成骨过程(28天和未分化的MSCs)中发挥关键作用。结论DNA复制和蛋白翻译在成骨早期都有功能，而溶酶体途径是成骨后期唯一与gcd相关的途径。gcd相关的核糖体途径占据了成骨的整个过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chronic Diseases and Translational Medicine Medicine-General Medicine

CiteScore

6.70

自引率

0.00%

发文量

195

审稿时长

35 weeks

期刊介绍： This journal aims to promote progress from basic research to clinical practice and to provide a forum for communication among basic, translational, and clinical research practitioners and physicians from all relevant disciplines. Chronic diseases such as cardiovascular diseases, cancer, diabetes, stroke, chronic respiratory diseases (such as asthma and COPD), chronic kidney diseases, and related translational research. Topics of interest for Chronic Diseases and Translational Medicine include Research and commentary on models of chronic diseases with significant implications for disease diagnosis and treatment Investigative studies of human biology with an emphasis on disease Perspectives and reviews on research topics that discuss the implications of findings from the viewpoints of basic science and clinical practic.