Plaque Microbiome in Caries-Active and Caries-Free Teeth by Dentition.

IF 2.2 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

JDR Clinical & Translational Research Pub Date : 2024-01-01 Epub Date: 2022-09-25 DOI:10.1177/23800844221121260

D Bhaumik, E Salzman, E Davis, F Blostein, G Li, K Neiswanger, R J Weyant, R Crout, D W McNeil, M L Marazita, B Foxman

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引用次数: 0

Abstract

Objective: Describe associations between dental caries and dental plaque microbiome, by dentition and family membership.

Methods: This cross-sectional analysis included 584 participants in the Center for Oral Health Research in Appalachia Cohort 1 (COHRA1). We sequenced the 16S ribosomal RNA gene (V4 region) of frozen supragingival plaque, collected 10 y prior, from 185 caries-active (enamel and dentinal) and 565 caries-free (no lesions) teeth using the Illumina MiSeq platform. Sequences were filtered using the R DADA2 package and assigned taxonomy using the Human Oral Microbiome Database.

Results: Microbiomes of caries-active and caries-free teeth were most similar in primary dentition and least similar in permanent dentition, but caries-active teeth were significantly less diverse than caries-free teeth in all dentition types. Streptococcus mutans had greater relative abundance in caries-active than caries-free teeth in all dentition types (P < 0.01), as did Veillonella dispar in primary and mixed dentition (P < 0.01). Fusobacterium sp. HMT 203 had significantly higher relative abundance in caries-free than caries-active teeth in all dentition types (P < 0.01). In a linear mixed model adjusted for confounders, the relative abundance of S. mutans was significantly greater in plaque from caries-active than caries-free teeth (P < 0.001), and the relative abundance of Fusobacterium sp. HMT 203 was significantly lower in plaque from caries-active than caries-free teeth (P < 0.001). Adding an effect for family improved model fit for Fusobacterium sp. HMT 203 but notS. mutans.

Conclusions: The diversity of supragingival plaque composition from caries-active and caries-free teeth changed with dentition, but S. mutans was positively and Fusobacterium sp. HMT 203 was negatively associated with caries regardless of dentition. There was a strong effect of family on the associations of Fusobacterium sp. HMT 203 with the caries-free state, but this was not true for S. mutans and the caries-active state.

Knowledge transfer statement: Patients' and dentists' concerns about transmission of bacteria within families causing caries should be tempered by the evidence that some shared bacteria may contribute to good oral health.

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龋病活跃牙和无龋牙牙菌斑微生物群（按牙期分类）。

目的：描述龋齿与牙菌斑微生物群之间的关系：描述龋齿与牙菌斑微生物群之间的关系，按牙齿和家庭成员分类：这项横断面分析包括阿巴拉契亚口腔健康研究中心队列 1（COHRA1）中的 584 名参与者。我们使用 Illumina MiSeq 平台对 10 年前收集的 185 颗龋活动牙（釉质和牙本质）和 565 颗无龋牙（无病变）的龈上牙菌斑的 16S 核糖体 RNA 基因（V4 区）进行了测序。使用 R DADA2 软件包过滤序列，并使用人类口腔微生物组数据库进行分类：结果：龋病活动牙和无龋牙的微生物组在初级牙列中最相似，在恒牙列中最不相似，但在所有牙列类型中，龋病活动牙的多样性明显低于无龋牙。在所有牙齿类型中，变异链球菌在龋活动牙中的相对丰度高于无龋牙（P < 0.01），Veillonella dispar在基牙和混合牙中的相对丰度也高于无龋牙（P < 0.01）。Fusobacterium sp. HMT 203 在所有牙列类型中的相对丰度明显高于无龋牙和活动牙（P < 0.01）。在根据混杂因素调整后的线性混合模型中，活动性龋齿牙菌斑中突变体的相对丰度明显高于无龋齿牙菌斑中突变体的相对丰度（P < 0.001），而活动性龋齿牙菌斑中镰刀菌属 HMT 203 的相对丰度明显低于无龋齿牙菌斑中镰刀菌属 HMT 203 的相对丰度（P < 0.001）。加入家族效应后，模型的拟合度提高了Fusobacterium sp：结论：龋病活动牙和无龋牙龈上牙菌斑成分的多样性会随牙齿状况而改变，但无论牙齿状况如何，变异杆菌与龋病呈正相关，而镰刀菌属 HMT 203 与龋病呈负相关。家庭对镰刀菌属 HMT 203 与无龋状态的相关性有很大影响，但对变异镰刀菌属和龋病活动状态的相关性则没有影响：患者和牙医对细菌在家庭中传播导致龋齿的担忧应根据一些共享细菌可能有助于良好口腔健康的证据而有所缓和。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

JDR Clinical & Translational Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

6.20

自引率

6.70%

发文量

期刊介绍： JDR Clinical & Translational Research seeks to publish the highest quality research articles on clinical and translational research including all of the dental specialties and implantology. Examples include behavioral sciences, cariology, oral & pharyngeal cancer, disease diagnostics, evidence based health care delivery, human genetics, health services research, periodontal diseases, oral medicine, radiology, and pathology. The JDR Clinical & Translational Research expands on its research content by including high-impact health care and global oral health policy statements and systematic reviews of clinical concepts affecting clinical practice. Unique to the JDR Clinical & Translational Research are advances in clinical and translational medicine articles created to focus on research with an immediate potential to affect clinical therapy outcomes.