A Case-Control Study to Determine the Microbiological Spectrum and Antibiotic Sensitivity Patterns in Congenital Nasolacrimal Duct Obstruction.

IF 1.2 Q3 OPHTHALMOLOGY
Journal of Current Ophthalmology Pub Date : 2022-07-26 eCollection Date: 2022-04-01 DOI:10.4103/joco.joco_216_21
Muthumeena Muthumalai, Md Shahid Alam, Neha Shrirao, B Mahalakshmi, Bipasha Mukherjee
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引用次数: 0

Abstract

Purpose: To analyze the microbiological spectrum and antibiotic sensitivity patterns in children with congenital nasolacrimal duct obstruction (CNLDO).

Methods: One hundred thirty-four eyes of 123 children in the age group of 0-16 years with a diagnosis of CNLDO who underwent lacrimal surgical procedures were included in this prospective comparative study. Sixty-two children in the age-matched group planned for intraocular surgery with patent nasolacrimal duct were deemed controls. The conjunctival swab after performing Regurgitation on Pressure over the Lacrimal Sac in the CNLDO group and the conjunctival swab in controls were sent for microbiological analysis. Antibiotic susceptibility testing was done for commonly employed antibiotics by the Kirby Bauer disk diffusion method.

Results: Of 134 samples collected in the CNLDO group, 111 (82.8%) samples were culture positive. There were 165 bacteria isolated, among which 139 (84.24% of isolates) were Gram-positive bacteria, and 26 (15.75% of isolates) were Gram-negative. Fungal isolates were obtained in 2.23% of cases. The most common Gram-positive isolate was Staphylococcus epidermidis (S. epidermidis) (n = 51, 30.9% of total isolates), and the most common Gram-negative isolate was Haemophilus influenza species (n = 9, 5.5% of total isolates). Gram-positive isolates were sensitive mostly to gentamicin and vancomycin (95.5% each), and Gram-negative isolates to amikacin (92.3%). Both Gram-positive and Gram-negative isolates were susceptible to gatifloxacin (80% each). Probing outcomes were similar among Gram-positive (success, 84.6%) and Gram-negative (success, 84.0%) organisms.

Conclusions: There was a predominance of Gram-positive isolates in children with CNLDO with S. epidermidis being the most common. The microbiological profile did not have any effect on the outcomes of probing.

Abstract Image

Abstract Image

先天性鼻泪管阻塞患者微生物谱及抗生素敏感性的病例对照研究。
目的:分析先天性鼻泪管梗阻(CNLDO)患儿的微生物谱及抗生素敏感性。方法:对123例年龄0 ~ 16岁诊断为CNLDO并行泪道手术的儿童134只眼进行前瞻性比较研究。年龄匹配组62例患儿计划行鼻泪管未闭眼内手术作为对照组。将CNLDO组泪囊加压反流后的结膜拭子和对照组的结膜拭子送去进行微生物学分析。采用Kirby Bauer纸片扩散法对常用抗生素进行药敏试验。结果:CNLDO组134份标本中,培养阳性111份(82.8%)。共分离细菌165株,其中革兰氏阳性菌139株(84.24%),革兰氏阴性菌26株(15.75%)。2.23%的病例分离出真菌。最常见的革兰氏阳性分离物为表皮葡萄球菌(S. epidermidis) (n = 51,占总分离物的30.9%),最常见的革兰氏阴性分离物为流感嗜血杆菌(n = 9,占总分离物的5.5%)。革兰氏阳性菌株对庆大霉素和万古霉素最敏感(各占95.5%),革兰氏阴性菌株对阿米卡星最敏感(占92.3%)。革兰氏阳性和革兰氏阴性菌株均对加替沙星敏感(各为80%)。革兰氏阳性菌(成功率为84.6%)和革兰氏阴性菌(成功率为84.0%)的探测结果相似。结论:CNLDO患儿以革兰氏阳性菌株为主,其中表皮葡萄球菌最为常见。微生物谱对探测结果没有任何影响。
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来源期刊
CiteScore
2.50
自引率
6.70%
发文量
45
审稿时长
8 weeks
期刊介绍: Peer Review under the responsibility of Iranian Society of Ophthalmology Journal of Current Ophthalmology, the official publication of the Iranian Society of Ophthalmology, is a peer-reviewed, open-access, scientific journal that welcomes high quality original articles related to vision science and all fields of ophthalmology. Journal of Current Ophthalmology is the continuum of Iranian Journal of Ophthalmology published since 1969.
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