Biological effect abundance analysis of hemolytic pathogens based on engineered biomimetic sensor

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2023-06-28 DOI:10.1016/j.bios.2023.115502

Qianqin Yuan , Dongsheng Mao , Xiaochen Tang , Chenbin Liu , Runchi Zhang , Jie Deng , Xiaoli Zhu , Wenxing Li , Qiuhong Man , Fenyong Sun

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引用次数: 0

Abstract

Conventional pathogen detection strategies based on the molecular structure or chemical characteristics of biomarkers can only provide the “physical abundance” of microorganisms, but cannot reflect the “biological effect abundance” in the true sense. To address this issue, we report an erythrocyte membrane-encapsulated biomimetic sensor cascaded with CRISPR-Cas12a (EMSCC). Taking hemolytic pathogens as the target model, we first constructed an erythrocyte membrane-encapsulated biomimetic sensor (EMS). Only hemolytic pathogens with biological effects can disrupt the erythrocyte membrane (EM), resulting in signal generation. Then the signal was amplified by cascading CRISPR-Cas12a, and more than 6.67 × 10⁴-fold improvement in detection sensitivity compared to traditional erythrocyte hemolysis assay was achieved. Notably, compared with polymerase chain reaction (PCR) or enzyme linked immunosorbent assay (ELISA)-based quantification methods, EMSCC can sensitively respond to the pathogenicity change of pathogens. For the detection of simulated clinical samples based on EMSCC, we obtained an accuracy of 95% in 40 samples, demonstrating its potential clinical value.

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基于工程仿生传感器的溶血病原体生物效应丰度分析

传统的基于生物标志物分子结构或化学特征的病原体检测策略只能提供微生物的“物理丰度”，而不能真正反映微生物的“生物效应丰度”。为了解决这个问题，我们报道了一种红细胞膜封装的与CRISPR-Cas12a级联的仿生传感器(EMSCC)。以溶血病原体为靶模型，首先构建了一种红细胞膜封装仿生传感器(EMS)。只有具有生物效应的溶血性病原体才能破坏红细胞膜，从而产生信号。然后通过CRISPR-Cas12a级联扩增信号，与传统红细胞溶血法相比，检测灵敏度提高6.67 × 104倍以上。值得注意的是，与基于聚合酶链反应(PCR)或酶联免疫吸附试验(ELISA)的定量方法相比，EMSCC能对病原体的致病性变化做出敏感的反应。对于基于EMSCC的模拟临床样本检测，我们在40个样本中获得了95%的准确率，显示了其潜在的临床价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.