Application of atomic force microscopy for investigation of Na(+),K(+)-ATPase signal-transducing function.

M M Khalisov, A V Ankudinov, V A Penniyaynen, D Dobrota, Boris V Krylov
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引用次数: 4

Abstract

The Young's modulus of 10-12-day-old chick embryos' sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low "endogenous" concentration of 10⁻¹⁰ M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na⁺,K⁺-ATPase signal-transducing function.

应用原子力显微镜研究Na(+)、K(+)- atp酶的信号转导功能。
利用PeakForce定量纳米机械图谱原子力显微镜测量了分离细胞培养的10-12日龄鸡胚感觉神经元的杨氏模量。在对照实验和应用瓦巴因后对天然细胞进行检测。在10⁻¹⁰M的低“内源性”浓度下,乌巴因倾向于增加感觉神经元的刚性。我们假设这种趋势是由于Na +、K + - atp酶信号转导功能的激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta physiologica Hungarica
Acta physiologica Hungarica 医学-生理学
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