M M Khalisov, A V Ankudinov, V A Penniyaynen, D Dobrota, Boris V Krylov
{"title":"Application of atomic force microscopy for investigation of Na(+),K(+)-ATPase signal-transducing function.","authors":"M M Khalisov, A V Ankudinov, V A Penniyaynen, D Dobrota, Boris V Krylov","doi":"10.1556/036.102.2015.2.2","DOIUrl":null,"url":null,"abstract":"<p><p>The Young's modulus of 10-12-day-old chick embryos' sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low \"endogenous\" concentration of 10⁻¹⁰ M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na⁺,K⁺-ATPase signal-transducing function.</p>","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.2.2","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta physiologica Hungarica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1556/036.102.2015.2.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
The Young's modulus of 10-12-day-old chick embryos' sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low "endogenous" concentration of 10⁻¹⁰ M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na⁺,K⁺-ATPase signal-transducing function.