Revisiting Protocols for the NMR Analysis of Bacterial Metabolomes.

Q4 Biochemistry, Genetics and Molecular Biology

Journal of Integrated OMICS Pub Date : 2013-12-01 DOI:10.5584/jiomics.v3i2.139

Steven Halouska, Bo Zhang, Rosmarie Gaupp, Shulei Lei, Emily Snell, Robert J Fenton, Raul G Barletta, Greg A Somerville, Robert Powers

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Abstract

Over the past decade, metabolomics has emerged as an important technique for systems biology. Measuring all the metabolites in a biological system provides an invaluable source of information to explore various cellular processes, and to investigate the impact of environmental factors and genetic modifications. Nuclear magnetic resonance (NMR) spectroscopy is an important method routinely employed in metabolomics. NMR provides comprehensive structural and quantitative information useful for metabolomics fingerprinting, chemometric analysis, metabolite identification and metabolic pathway construction. A successful metabolomics study relies on proper experimental protocols for the collection, handling, processing and analysis of metabolomics data. Critically, these protocols should eliminate or avoid biologically-irrelevant changes to the metabolome. We provide a comprehensive description of our NMR-based metabolomics procedures optimized for the analysis of bacterial metabolomes. The technical details described within this manuscript should provide a useful guide to reliably apply our NMR-based metabolomics methodology to systems biology studies.

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重温细菌代谢组核磁共振分析程序

在过去十年中，代谢组学已成为系统生物学的一项重要技术。测量生物系统中的所有代谢物为探索各种细胞过程、研究环境因素和基因修饰的影响提供了宝贵的信息来源。核磁共振（NMR）光谱是代谢组学中经常使用的一种重要方法。核磁共振可提供全面的结构和定量信息，有助于代谢组学指纹图谱、化学计量分析、代谢物鉴定和代谢途径构建。成功的代谢组学研究有赖于收集、处理、加工和分析代谢组学数据的正确实验方案。至关重要的是，这些方案应消除或避免代谢组发生与生物学无关的变化。我们全面介绍了为分析细菌代谢组而优化的基于核磁共振的代谢组学程序。本手稿中描述的技术细节应能为将我们基于核磁共振的代谢组学方法可靠地应用于系统生物学研究提供有用的指导。

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来源期刊

Journal of Integrated OMICS Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： JIOMICS provides a forum for the publication of original research papers, letters to the editor, short communications, and critical reviews in all branches of pure and applied –omics subjects, such as proteomics, metabolomics, metallomics and genomics. Especial interest is given to papers where more than one –omics subject is covered. Papers are evaluated based on scientific novelty and demonstrated scientific applicability. Original research papers on fundamental studies, and novel sensor and instrumentation development, are especially encouraged. Novel or improved findings in areas such as clinical, medicinal, biological, environmental and materials –omics are welcome.