Brassinosteroid insensitive 1-associated kinase 1 (OsI-BAK1) is associated with grain filling and leaf development in rice

IF 4 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2015-06-15 DOI:10.1016/j.jplph.2015.05.003

Choy-Yuen Khew , Chin-Jit Teo , Wai-Sun Chan , Hann-Ling Wong , Parameswari Namasivayam , Chai-Ling Ho

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引用次数: 24

Abstract

Brassinosteroid Insensitive 1 (BRI1)-Associated Kinase I (BAK1) has been reported to interact with BRI1 for brassinosteroid (BR) perception and signal transduction that regulate plant growth and development. The aim of this study is to investigate the functions of a rice OsBAK1 homologue, designated as OsI-BAK1, which is highly expressed after heading. Silencing of OsI-BAK1 in rice plants produced a high number of undeveloped green and unfilled grains compared to the untransformed plants. Histological analyses demonstrated that embryos were either absent or retarded in their development in these unfilled rice grains of OsI-BAK1 RNAi plants. Down regulation of OsI-BAK1 caused a reduction in cell number and enlargement in leaf bulliform cells. Furthermore, transgenic rice plants overexpressing OsI-BAK1 were demonstrated to have corrugated and twisted leaves probably due to increased cell number that caused abnormal bulliform cell structure which were enlarged and plugged deep into leaf epidermis. The current findings suggest that OsI-BAK1 may play an important role in the developmental processes of rice grain filling and leaf cell including the bulliform cells.

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油菜素内酯不敏感1-相关激酶1 (si - bak1)与水稻籽粒灌浆和叶片发育有关

据报道，brassinosteroids Insensitive 1 (BRI1)-Associated Kinase I (BAK1)与BRI1相互作用，参与调控植物生长发育的brassinosteroids (BR)感知和信号转导。本研究的目的是研究水稻OsBAK1同源物OsI-BAK1的功能，该同源物在抽穗后高度表达。与未转化的水稻植株相比，si - bak1基因的沉默产生了大量未发育的绿粒和未灌浆的籽粒。组织学分析表明，在这些si - bak1 RNAi植株的未填充稻谷中，胚胎缺失或发育迟缓。下调OsI-BAK1的表达可导致叶片球状细胞数量减少、细胞增大。此外，过表达si - bak1的转基因水稻植株叶片呈波纹状和扭曲状，这可能是由于细胞数量增加导致异常的球泡状细胞结构扩大并深入叶表皮所致。目前的研究结果表明，OsI-BAK1可能在水稻籽粒灌浆和叶片细胞包括球状细胞的发育过程中发挥重要作用。

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.