Fabrication of CeVO4 as nanozyme for facile colorimetric discrimination of hydroquinone from resorcinol and catechol

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2017-08-01 DOI:10.1016/j.snb.2017.03.042

Haiguan Yang, Junqi Zha, Peng Zhang, Yuemei Qin, Tao Chen, Fanggui Ye

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引用次数: 57

Abstract

A simple method was proposed for the preparation of CeVO₄. The as-synthesized CeVO₄ was, for the first time, demonstrated to exhibit both peroxidase-like and oxidase-like activity, which catalyzes the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to form a typical blue solution in the presence or absence of H₂O₂. Moreover, the mechanism of its dual-enzyme activity was investigated in detail. The Michaelis constant (K_m) value for CeVO₄ (0.136 mM) was lower than that of horseradish peroxidase (0.424 mM) with TMB as the substrate. Interestingly, hydroquinone (H₂Q), dihydroxybenzene isomer, undergoes reduction accompanying the oxidation of TMB by the CeVO₄ oxidase mimic along with a visible color change, while the other two dihydroxybenzene isomers, i.e., resorcinol (RC) and catechol (CC), do not. Based on these findings, a colorimetric platform was developed to discriminate H₂Q from RC and CC. Under optimal conditions, a linear relationship between the H₂Q concentration and absorbance was observed from 0.05 to 8 μM, and a limit of detection of 0.04 μM was achieved. Moreover, this colorimetric platform can selectively reveal H₂Q concentrations in the presence of other dihydroxybenzene isomers.

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CeVO4纳米酶的制备及其对苯二酚和儿茶酚的比色鉴别

提出了一种制备CeVO4的简便方法。本文首次证明了CeVO4具有过氧化物酶和氧化酶样活性，在H2O2存在或不存在的情况下，CeVO4都能催化3,3 '，5,5 ' -四甲基联苯胺(TMB)氧化形成典型的蓝色溶液。并对其双酶活性的机理进行了详细的探讨。CeVO4的米切里斯常数(Km)值(0.136 mM)低于以TMB为底物的辣根过氧化物酶(0.424 mM)。有趣的是，对苯二酚异构体(H2Q)在CeVO4氧化酶模拟物氧化TMB的过程中会发生还原，并伴有可见的颜色变化，而其他两种二羟基苯异构体，即间苯二酚(RC)和儿茶酚(CC)则没有。在此基础上，建立了H2Q与RC和CC的比色平台，在最优条件下，H2Q浓度与吸光度在0.05 ~ 8 μM范围内呈线性关系，检出限为0.04 μM。此外，该比色平台可以选择性地显示其他二羟基苯异构体存在时的H2Q浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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