Protective effect of proteins derived from Calotropis procera latex against acute inflammation in rat

V. L. Kumar, B. Guruprasad, P. Chaudhary, S. M. A. Fatmi, R. S. B. Oliveira, M. V. Ramos
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引用次数: 19

Abstract

  1. The non-dialysable proteins present in the latex of plant Calotropis procera possess anti-inflammatory and analgesic properties.
  2. The aim of this study was to evaluate the effect of latex proteins (LP) on the level of inflammatory mediators, oxidative stress markers and tissue histology in the rat model of carrageenan-induced acute inflammation. This study also aimed at evaluating the anti-inflammatory efficacy of LP against different mediators and comparing it with their respective antagonists.
  3. Paw inflammation was induced by subplantar injection of carrageenan, and the effect of LP was evaluated on oedema volume, level of TNF-α, PGE2, myeloperoxidase, nitric oxide, reduced glutathione, thiobarbituric acid-reactive substances and tissue histology at the time of peak inflammation.
  4. Paw inflammation was also induced by histamine, serotonin, bradykinin and PGE2, and the inhibitory effect of LP against these mediators was compared with their respective antagonists at the time of peak effect.
  5. Treatment with LP produced a dose-dependent inhibition of oedema formation, and its anti-inflammatory effect against carrageenan-induced paw inflammation was accompanied by reduction in the levels of inflammatory mediators, oxidative stress markers and normalization of tissue architecture.
  6. LP also produced a dose-dependent inhibition of oedema formation induced by different inflammatory mediators, and its efficacy was comparable to their respective antagonists and more pronounced than that of diclofenac.
  7. Thus, our study shows that LP has a potential to be used for the treatment of various inflammatory conditions where the role of these mediators is well established.
原角鹿乳蛋白对大鼠急性炎症的保护作用
植物原角鹿角乳乳中的非透析蛋白具有抗炎和镇痛的特性。本研究旨在探讨乳胶蛋白(latex protein, LP)对卡拉胶致急性炎症大鼠模型炎症介质、氧化应激标志物及组织组织学的影响。本研究还旨在评估LP对不同介质的抗炎作用,并将其与各自的拮抗剂进行比较。通过足底注射角叉菜胶诱导足跖炎症,观察LP对炎症峰值时足跖水肿量、TNF-α、PGE2、髓过氧化物酶、一氧化氮、还原性谷胱甘肽、硫代巴比妥酸反应性物质水平及组织组织学的影响。组胺、5 -羟色胺、缓激肽和PGE2也可诱导足跖炎症,比较LP对这些介质的抑制作用及其拮抗剂在作用峰值时的抑制作用。LP治疗对水肿形成具有剂量依赖性的抑制作用,其抗卡拉胶诱导的足跖炎症的抗炎作用伴随着炎症介质、氧化应激标志物水平的降低和组织结构的正常化。LP对不同炎症介质诱导的水肿形成也有剂量依赖性的抑制作用,其效果与各自的拮抗剂相当,比双氯芬酸更明显。因此,我们的研究表明,LP具有用于治疗各种炎症条件的潜力,其中这些介质的作用已经得到了很好的确立。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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