Effects of adnexal torsion on the Fallopian tube in rats: a histologic and immunohistochemical study.

Abstract

Objective: To investigate histopathological and immunohistochemical changes in the Fallopian tube structure following ovarian torsion.

Study design: Thirty fertile, female, adult Wistar albino rats, weighing 200-220 g, were randomly divided into 3 equal groups (n = 10): sham, torsion, and detorsion. In the torsion and detorsion groups, bilateral adnexal torsion (3-hour ischemia) was carried out. Bilateral adnexal detorsion (3-hour reperfusion) was performed in the detorsion group. Fallopian tube sections were stained hematoxylin and eosin, periodic acid-Schiff, Mallory-Azan and immunohistochemically-stained desmin, α smooth actin, and CD68 antibodies.

Results: In the sections of the ischemic group, degeneration of epithelium, loss of cilia, dilation of blood vessels, and hemorrhages were observed. Image analysis of the studied Fallopian section revealed a significant decrease in density of desmin in the torsion group. Moreover, strong positive cytoplasmic CD68 expression was observed in the torsion group.

Conclusion: The use of effective antiinflammatory treatments may contribute to the conservative surgery in preservation of ovarian reserve and tubal structure.