In vitro studies of the toxic effects of silver nanoparticles on HeLa and U937 cells.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2015-03-05 eCollection Date: 2015-01-01 DOI:10.2147/NSA.S78134
Said I Kaba, Elena M Egorova
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引用次数: 0

Abstract

In the last decade, much attention has been paid to studies of the effect of silver nanoparticles (Ag NPs) on tumor cells. Apart from elucidation of the mechanism of NPs' interaction with mammalian cells, these studies are aimed at discovering new effective antitumor drugs. In this work, we report about the toxic effects of Ag NPs observed on two types of tumor cells: HeLa (adhesive cells) and U937 (suspension cells). The Ag NPs were obtained by an original method of biochemical synthesis. Particle size was 13.2±4.72 nm, and zeta potential was -61.9±3.2 mV. The toxicity of Ag NPs in the concentration range 0.5-8.0 μg Ag/mL was determined by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cytofluorometry after 4 and 24 hours' incubation. It was found that Ag NPs had high toxicity toward both cell types. The minimal concentrations where a toxicity effect was registered (toxicity thresholds) lied in the range 0.5-2.0 μg Ag/mL. In parallel with the Ag NP solution, cells were incubated with water solutions of the NP stabilizer (aerosol-OT) and Ag(+) ions (as silver nitrate). It was shown that aerosol-OT had no effect on the viability on HeLa cells, but was moderately toxic toward U937, though less dangerous for these cells than Ag NPs. With Ag(+) ions, for HeLa no toxic effect was observed, while for U937 they were as toxic as the Ag NPs. The data obtained indicate that Ag NPs as used in this study may prove to be useful for the creation of medicines for cancer therapy.

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银纳米粒子对 HeLa 和 U937 细胞毒性作用的体外研究。
近十年来,银纳米粒子(Ag NPs)对肿瘤细胞影响的研究备受关注。除了阐明 NPs 与哺乳动物细胞相互作用的机制外,这些研究还旨在发现新的有效抗肿瘤药物。在这项工作中,我们报告了 Ag NPs 对两种肿瘤细胞的毒性作用:HeLa(粘附细胞)和 U937(悬浮细胞)。Ag NPs 是通过独创的生化合成方法获得的。粒径为 13.2±4.72 nm,zeta 电位为 -61.9±3.2 mV。通过 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 法和细胞荧光测定法,测定了培养 4 小时和 24 小时后 Ag NPs 在 0.5-8.0 μg Ag/mL 浓度范围内的毒性。结果发现,Ag NPs 对这两种细胞都有很高的毒性。出现毒性效应的最小浓度(毒性阈值)范围为 0.5-2.0 μg Ag/mL。在用 Ag NP 溶液培养细胞的同时,还用 NP 稳定剂(气溶胶-OT)和 Ag(+)离子(硝酸银)的水溶液培养细胞。结果表明,气溶胶-OT 对 HeLa 细胞的存活率没有影响,但对 U937 有中度毒性,不过对这些细胞的危害小于 Ag NPs。Ag(+) 离子对 HeLa 细胞没有毒性影响,而对 U937 细胞的毒性则与 Ag NPs 相当。所获得的数据表明,本研究中使用的 Ag NPs 有可能被证明有助于制造治疗癌症的药物。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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