Vascular leakage in dengue hemorrhagic Fever is associated with dengue infected monocytes, monocyte activation/exhaustion, and cytokines production.

IF 2.5 Q2 PERIPHERAL VASCULAR DISEASE
International Journal of Vascular Medicine Pub Date : 2015-01-01 Epub Date: 2015-02-05 DOI:10.1155/2015/917143
Sirichan Chunhakan, Punnee Butthep, Sutee Yoksan, Kanchana Tangnararatchakit, Ampaiwan Chuansumrit
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引用次数: 31

Abstract

The vascular leakage was shown by the increment of hematocrit (Hct), dengue viral infected monocyte, monocyte status, and cytokines production in patients infected with dengue virus. Dengue viral antigens were demonstrated in monocytes (CD14+) from peripheral blood mononuclear cells. The increased levels of Hct, interleukin- (IL-) 10, and tumor necrosis factor-alpha (TNF-α) were detected in dengue fever (DF), dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) patients as compared with other febrile illnesses (OFIs). The highest levels of Hct and IL-10 were detected in DSS patients as compared with other groups (P < 0.05) especially on one day before and after defervescence. The unstimulated and lipopolysaccharide- (LPS-) stimulated monocytes from DSS patients showed the significantly decreased of intracellular IL-1β and TNF-α. In addition, the lowest level of mean fluorescence intensity (MFI) of CD11b expression on monocytes surface in DSS patients was also demonstrated. Furthermore, the negative correlations between IL-10 levels and intracellular IL-1β and MFI of CD11b expression in unstimulated and LPS-stimulated monocytes were also detected. Nevertheless, not only were the relationships between the prominent IL-10 and the suppression of intracellular monocyte secretion, namely, IL-1β, TNF-α, demonstrated but also the effect of vascular leakage was observed.

Abstract Image

Abstract Image

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登革出血热的血管渗漏与登革感染单核细胞、单核细胞激活/衰竭和细胞因子产生有关。
血管渗漏表现为登革热病毒感染患者红细胞压积(Hct)的增加、登革热病毒感染的单核细胞、单核细胞状态和细胞因子的产生。登革热病毒抗原在外周血单核细胞(CD14+)中被证实。与其他发热性疾病(OFIs)相比,登革热(DF)、登革出血热(DHF)和登革休克综合征(DSS)患者的Hct、白细胞介素- (IL-) 10和肿瘤坏死因子-α (TNF-α)水平升高。DSS患者的Hct和IL-10水平与其他组比较,差异有统计学意义(P < 0.05),特别是在退热前后1 d。未刺激和脂多糖(LPS)刺激的DSS患者单核细胞显示细胞内IL-1β和TNF-α显著降低。此外,DSS患者单核细胞表面CD11b的平均荧光强度(MFI)最低。此外,在未刺激和lps刺激的单核细胞中,IL-10水平与细胞内IL-1β和CD11b表达的MFI呈负相关。然而,不仅证明了IL-10的显著升高与抑制细胞内单核细胞分泌(即IL-1β、TNF-α)之间的关系,而且还观察到了血管渗漏的影响。
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来源期刊
International Journal of Vascular Medicine
International Journal of Vascular Medicine PERIPHERAL VASCULAR DISEASE-
CiteScore
3.50
自引率
0.00%
发文量
7
审稿时长
16 weeks
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