Richard K Zimmerman, Charles R Rinaldo, Mary Patricia Nowalk, G K Balasubramani, Mark G Thompson, Arlene Bullotta, Michael Susick, Stephen Wisniewski
{"title":"Detection of influenza virus infection using two PCR methods.","authors":"Richard K Zimmerman, Charles R Rinaldo, Mary Patricia Nowalk, G K Balasubramani, Mark G Thompson, Arlene Bullotta, Michael Susick, Stephen Wisniewski","doi":"10.1155/2014/274679","DOIUrl":null,"url":null,"abstract":"<p><p>Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses. </p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2014 ","pages":"274679"},"PeriodicalIF":1.1000,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/274679","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2014/274679","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2014/12/9 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 7
Abstract
Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.
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