Estrogen receptor alpha and androgen receptor are commonly expressed in well-differentiated liposarcoma.

Q2 Medicine
BMC Clinical Pathology Pub Date : 2014-10-22 eCollection Date: 2014-01-01 DOI:10.1186/1472-6890-14-42
Davis R Ingram, Lloye M Dillon, Dina Chelouche Lev, Alexander Lazar, Elizabeth G Demicco, Burton L Eisenberg, Todd W Miller
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引用次数: 10

Abstract

Background: Liposarcoma (LS) is the second-most common type of soft-tissue sarcoma. Despite advances in knowledge and treatment of this disease, there remains a need for more effective LS therapy. Steroid hormone receptors regulate metabolism in adipocytes. Estrogen receptor alpha (ER), progesterone receptor (PR), and androgen receptor (AR) have been implicated in the pathophysiology of other cancer types. We sought to comprehensively determine temporal expression patterns of these receptors in LS.

Methods: We analyzed 561 histologically subtyped LS specimens from 354 patients for expression of ER, PR, and AR by immunohistochemistry (IHC) using diagnostic-grade reagents and protocols. The fractions of positively stained tumor cells were scored within each specimen. IHC scores were compared across LS subtypes using the Kruskal-Wallis test, and subtypes were compared using Dunn's post-hoc test. Ages of patients with receptor-positive vs. -negative LS were compared by t-test. Genders and races were compared for hormone receptor positivity using Fisher's exact test and Chi-square analysis, respectively. Recurrence-free survival was compared between receptor-positive and negative patients by log-rank test. p< 0.05 was considered significant.

Results: ER and AR were frequently expressed in LS, while few tumors expressed PR. Most of the ER + and AR + samples were of the well-differentiated LS subtype. A smaller fraction of de-differentiated LS expressed ER or AR, but expression was common within well-differentiated regions of tumors histologically classified as de-differentiated LS. In LS specimens from patients who underwent multiple surgeries over time, receptor expression frequently changed over time, which may be attributable in part to intratumor heterogeneity, varying degrees of de-differentiation, and biopsy bias. ER and AR were frequently co-expressed. Receptor status was not significantly associated with gender or race, but AR and PR expression were associated with earlier age at diagnosis. Receptor expression was not associated with altered recurrence-free survival.

Conclusions: ER and AR are commonly expressed in LS, particularly in well-differentiated tumors. These data warrant further functional study to determine receptor function in LS, and the potential efficacy of anti-hormone therapies for the treatment of patients with LS.

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雌激素受体α和雄激素受体在高分化脂肪肉瘤中普遍表达。
背景:脂肪肉瘤(LS)是第二常见的软组织肉瘤类型。尽管这种疾病的知识和治疗取得了进展,但仍然需要更有效的LS治疗。类固醇激素受体调节脂肪细胞的代谢。雌激素受体(ER)、孕激素受体(PR)和雄激素受体(AR)参与了其他类型癌症的病理生理。我们试图全面确定这些受体在LS中的时间表达模式。方法:采用诊断级试剂和方案,通过免疫组化(IHC)分析354例LS患者的561例组织学亚型标本中ER、PR和AR的表达。对每个标本中阳性染色的肿瘤细胞进行评分。使用Kruskal-Wallis测试比较不同LS亚型的IHC评分,使用Dunn事后测试比较不同亚型的IHC评分。采用t检验比较受体阳性和受体阴性LS患者的年龄。分别使用Fisher精确检验和卡方分析比较性别和种族的激素受体阳性。通过log-rank检验比较受体阳性和阴性患者的无复发生存率。结果:LS中ER和AR表达较多,PR表达较少,ER +和AR +多为LS高分化亚型。较小比例的去分化LS表达ER或AR,但在组织学上被分类为去分化LS的肿瘤的高分化区域中表达是常见的。在长期接受多次手术的LS患者标本中,受体表达经常随时间变化,这可能部分归因于肿瘤内异质性、不同程度的去分化和活检偏倚。ER和AR频繁共表达。受体状态与性别或种族无显著相关性,但AR和PR表达与早期诊断年龄相关。受体表达与无复发生存率的改变无关。结论:ER和AR在LS中普遍表达,尤其是在高分化肿瘤中。这些数据支持进一步的功能研究,以确定受体在LS中的功能,以及抗激素疗法治疗LS患者的潜在疗效。
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来源期刊
BMC Clinical Pathology
BMC Clinical Pathology Medicine-Pathology and Forensic Medicine
CiteScore
3.30
自引率
0.00%
发文量
0
期刊介绍: BMC Clinical Pathology is an open access journal publishing original peer-reviewed research articles in all aspects of histopathology, haematology, clinical biochemistry, and medical microbiology (including virology, parasitology, and infection control). BMC Clinical Pathology (ISSN 1472-6890) is indexed/tracked/covered by PubMed, CAS, EMBASE, Scopus and Google Scholar.
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