Involvement of retrotransposon L1 in stemness and cellular plasticity.

Q2 Biochemistry, Genetics and Molecular Biology

Cell Communication and Adhesion Pub Date : 2015-01-01 Epub Date: 2014-10-20 DOI:10.3109/15419061.2014.970270

Panagiotis Apostolou, Maria Toloudi, Marina Chatziioannou, Eleni Kourtidou, Georgia Mimikakou, Ioanna Vlachou, Aikaterini Chlichlia, Ioannis Papasotiriou

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引用次数: 9

Abstract

Epithelial-to-mesenchymal transition (EMT) as well as the reverse process, mesenchymal-to-epithelial transition (MET) is important during embryogenesis. EMT is also involved in cancer invasion and metastasis, and can generate cells with properties similar to those of stem cells. Retrotransposons can rearrange the genome by inserting DNA in new loci, thus inducing mutations. This study examines the gene expression of transcription factors involved in EMT and MET. In the second experimental panel, the gene expression of L1 retrotransposon was studied. L1-open reading frame (ORF) 2 mRNA was found to be expressed both in cancer and cancer stem cells, while L1-ORF1 mRNA was expressed only in cancer cells. The suppression of L1-ORF2 gene expression demonstrated that this retrotransposon might affect EMT in colon cancer stem cells. This study highlights that the EMT process seems to differ between cancer cells and cancer stem cells, and that transposable elements seem to be involved in the process, influencing cellular plasticity.

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反转录转座子L1参与干细胞和细胞可塑性。

上皮-间充质转化(EMT)以及相反的过程，间充质-上皮转化(MET)在胚胎发生过程中很重要。EMT还参与癌症的侵袭和转移，并能产生具有类似干细胞特性的细胞。反转录转座子可以通过在新的位点插入DNA来重新排列基因组，从而诱导突变。本研究探讨了EMT和MET相关转录因子的基因表达。在第二组实验中，我们研究了L1反转录转座子的基因表达。L1-open reading frame (ORF) 2 mRNA在肿瘤细胞和肿瘤干细胞中均有表达，而L1-ORF1 mRNA仅在癌细胞中表达。L1-ORF2基因表达的抑制表明该反转录转座子可能影响结肠癌干细胞的EMT。这项研究强调了癌细胞和癌症干细胞之间的EMT过程似乎不同，并且转座因子似乎参与了该过程，影响细胞的可塑性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Communication and Adhesion 生物-生化与分子生物学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cessation Cell Communication and Adhesion is an international Open Access journal which provides a central forum for research on mechanisms underlying cellular signalling and adhesion. The journal provides a single source of information concerning all forms of cellular communication, cell junctions, adhesion molecules and families of receptors from diverse biological systems. The journal welcomes submission of original research articles, reviews, short communications and conference reports.