Ratiometric fluorescence detection of tetracycline for tetracycline adjuvant screening in bacteria

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2022-12-01 DOI:10.1016/j.snb.2022.132687

Shangqing Zhang, Qixuan Sun, Xun Liu, Haiyan Li, Jianhua Wang, Mingli Chen

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引用次数: 6

Abstract

Persistent generation of tetracycline (TC) resistant bacteria poses serious risks for public health. It is of great importance to develop a strategy for rapid screening TC adjuvants for restore TC-susceptibility against resistant bacteria. Herein, a flower-like europium-doped nanoprobe is created by coordinating Eu³⁺ with adenine (Ade)-graphitic carbon nitride (g-C₃N₄) conjugate (g-C₃N₄-Ade-Eu). TC quenches the intrinsic fluorescence of g-C₃N₄ at 436 nm via internal filter effect (IFE), and meanwhile the antenna effect (AE) of TC significantly promotes the emission of Eu³⁺ at 618 nm. These fluorescence responsive behaviors of g-C₃N₄-Ade-Eu ensure ratiometric fluorescence assay of TC within 0.01 − 10 μM, along with a limit of detection (LOD) of 5.0 nM. The nanoflower structure exhibits large surface area, which endows enrichment of TC on the flower surface to ensure a high assay sensitivity. Tetracycline in bacteria is quantified for the first time by this protocol, and it further facilitates the rapid screening (∼3 h) of tetracycline adjuvants in E.coli ER2738.

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四环素比例荧光检测在细菌中筛选四环素佐剂的应用

四环素耐药细菌的持续产生对公众健康构成严重威胁。开发一种快速筛选TC佐剂的策略对于恢复TC对耐药菌的敏感性具有重要意义。本文将Eu3+与腺嘌呤(Ade)-石墨氮化碳(g-C3N4)偶联物(g-C3N4- de- eu)配位，制备了一种花状掺铕纳米探针。TC通过内部滤光效应(IFE)淬灭了g-C3N4在436 nm处的本征荧光，同时TC的天线效应(AE)显著促进了618 nm处Eu3+的发射。g- c3n4 - de- eu的这些荧光响应行为确保了TC的比例荧光检测在0.01 ~ 10 μM范围内，检出限(LOD)为5.0 nM。纳米花结构具有较大的表面积，这使得TC在花表面的富集确保了高的检测灵敏度。该方案首次定量了细菌中的四环素，并进一步促进了大肠杆菌ER2738中四环素佐剂的快速筛选(~ 3 h)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.