Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α

IF 3.261

Journal of Photochemistry and Photobiology Pub Date : 2022-12-01 DOI:10.1016/j.jpap.2022.100150

Chi Zhang , Lili Xu , Mizuki Endo , Tomoaki Kahyo , Kenji Kikushima , Makoto Horikawa , Makoto Murakami , A.S.M. Waliullah , Md.Mahmudul Hasan , Takumi Sakamoto , Yutaka Takahashi , Shuhei Aramaki , Takeaki Ozawa , Mitsutoshi Setou

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引用次数: 0

Abstract

Light, an inevitable factor for human life, can have negative effects such as cellular inflammation and thus be toxic to cells. Lipids are important biomolecules that are involved in cellular inflammatory responses. Our research revealed the impact of light on cellular lipids. We discovered that blue light irradiation altered cellular lipidome based on non-targeted lipidomic analysis. Inflammation-related lysophospholipids, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic acid, in particular, were significantly increased. We created opto-PiC, an optolipidomic tool based on the optogenetically engineered cPLA2 enzyme. Light-induced lipidomic changes were reversed by opto-PiC. Furthermore, the results showed that opto-PiC reduced cell death rate under blue light. Overall, our research provided lipidomic insights and optolipidomics approach into light toxicity. This could help the therapy development in the future for inflammation-related disease.

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蓝光改变细胞脂质组-光基因工程cPLA2α可以调节光诱导的脂质组变化

光是人类生命不可避免的因素，但也会产生细胞炎症等负面影响，从而对细胞产生毒性。脂质是参与细胞炎症反应的重要生物分子。我们的研究揭示了光对细胞脂质的影响。基于非靶向脂质组学分析，我们发现蓝光照射改变了细胞脂质组。尤其是与炎症相关的溶血磷脂、花生四烯酸、二十二碳六烯酸和二十碳五烯酸显著增加。我们创建了opto-PiC，一种基于光基因工程cPLA2酶的光脂组学工具。光诱导的脂质组改变被opto-PiC逆转。此外，结果表明，opto-PiC降低了蓝光下细胞的死亡率。总的来说，我们的研究为光毒性提供了脂质组学的见解和光质组学的方法。这可能有助于未来炎症相关疾病的治疗发展。

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来源期刊

Journal of Photochemistry and Photobiology

CiteScore

4.10

自引率

0.00%

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