Microanatomical Study of Embryonic Gonadal Development in Japanese Quail (Coturnix japonica).

Anatomy research international Pub Date : 2014-01-01 Epub Date: 2014-09-03 DOI:10.1155/2014/168614

Sittipon Intarapat, Orawan Satayalai

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引用次数: 12

Abstract

Gonadal development of quail embryos was examined histologically using histological and histochemical methods. In the present study, quail embryos were studied at various stages of incubation period based on phases of gonadogenesis. Germ cell migration was observed on day 3-4 but gonadal differentiation and gonadal function were observed on day 6-8 and day 11-14, respectively. During germ cell migration, quail primordial germ cells (qPGCs) were successfully detected in both left and right genital ridges as well as the dorsal mesentery by lectin histochemistry. Unexpectedly, qPGCs-like cells were found next to the neural tube by Mallory-AZAN stain. During gonadal differentiation, embryonic sex can be distinguished histologically since day 8 of incubation. Embryonic testis exhibited a thin cortex, whereas embryonic ovary exhibited a thick cortex. Testicular cord formation was found in the medulla of embryonic testes while the lacunae and fat-laden cells were found in the medulla of embryonic ovary during gonadal function. This is the first report on a comparison of phases of gonadogenesis and histochemical study of quail embryonic gonads in both sexes.

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日本鹌鹑胚胎性腺发育的显微解剖研究。

采用组织学和组织化学方法对鹌鹑胚胎性腺发育进行组织学观察。在本研究中，研究了鹌鹑胚胎在不同的孵育期的性腺发育阶段。第3-4天观察生殖细胞迁移，第6-8天和第11-14天分别观察性腺分化和性腺功能。在生殖细胞迁移过程中，通过凝集素组织化学方法成功检测到鹌鹑的左右生殖脊和背肠系膜中存在原始生殖细胞(qPGCs)。出乎意料的是，通过Mallory-AZAN染色，在神经管旁发现了qpgcs样细胞。在性腺分化过程中，从孵化第8天开始，组织学上就可以区分胚胎性别。胚胎睾丸皮层薄，而胚胎卵巢皮层厚。在性腺功能期间，胚胎睾丸髓质中发现睾丸索形成，胚胎卵巢髓质中发现腔隙和脂肪细胞。本文首次报道了鹌鹑两性胚胎性腺性腺发育阶段的比较和组织化学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Anatomy research international

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