Prevention of bacterial foodborne disease using nanobiotechnology.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2014-08-25 eCollection Date: 2014-01-01 DOI:10.2147/NSA.S51101
Craig Billington, J Andrew Hudson, Elaine D'Sa
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引用次数: 0

Abstract

Foodborne disease is an important source of expense, morbidity, and mortality for society. Detection and control constitute significant components of the overall management of foodborne bacterial pathogens, and this review focuses on the use of nanosized biological entities and molecules to achieve these goals. There is an emphasis on the use of organisms called bacteriophages (phages: viruses that infect bacteria), which are increasingly being used in pathogen detection and biocontrol applications. Detection of pathogens in foods by conventional techniques is time-consuming and expensive, although it can also be sensitive and accurate. Nanobiotechnology is being used to decrease detection times and cost through the development of biosensors, exploiting specific cell-recognition properties of antibodies and phage proteins. Although sensitivity per test can be excellent (eg, the detection of one cell), the very small volumes tested mean that sensitivity per sample is less compelling. An ideal detection method needs to be inexpensive, sensitive, and accurate, but no approach yet achieves all three. For nanobiotechnology to displace existing methods (culture-based, antibody-based rapid methods, or those that detect amplified nucleic acid) it will need to focus on improving sensitivity. Although manufactured nonbiological nanoparticles have been used to kill bacterial cells, nanosized organisms called phages are increasingly finding favor in food safety applications. Phages are amenable to protein and nucleic acid labeling, and can be very specific, and the typical large "burst size" resulting from phage amplification can be harnessed to produce a rapid increase in signal to facilitate detection. There are now several commercially available phages for pathogen control, and many reports in the literature demonstrate efficacy against a number of foodborne pathogens on diverse foods. As a method for control of pathogens, nanobiotechnology is therefore flourishing.

Abstract Image

利用纳米生物技术预防细菌性食源性疾病。
食源性疾病是社会开支、发病率和死亡率的重要来源。检测和控制是食源性细菌病原体整体管理的重要组成部分,本综述将重点讨论如何利用纳米生物实体和分子来实现这些目标。重点介绍被称为噬菌体(噬菌体:感染细菌的病毒)的生物体的使用,这种生物体正越来越多地用于病原体检测和生物控制应用。用传统技术检测食品中的病原体既费时又费钱,尽管这种方法也很灵敏和准确。纳米生物技术正通过开发生物传感器,利用抗体和噬菌体蛋白的特定细胞识别特性,缩短检测时间,降低检测成本。虽然每次检测的灵敏度可能很高(例如,检测一个细胞),但检测的体积非常小,这意味着每个样本的灵敏度不那么令人信服。理想的检测方法必须成本低廉、灵敏度高、准确性好,但目前还没有一种方法能同时做到这三点。纳米生物技术要取代现有方法(基于培养基、基于抗体的快速方法或检测扩增核酸的方法),就必须把重点放在提高灵敏度上。尽管人造非生物纳米粒子已被用于杀死细菌细胞,但被称为噬菌体的纳米生物体在食品安全应用中越来越受到青睐。噬菌体可进行蛋白质和核酸标记,具有很强的特异性,噬菌体扩增产生的典型大 "爆发尺寸 "可用于产生快速增加的信号,以便于检测。目前,市面上有几种用于病原体控制的噬菌体,许多文献报告显示,它们对不同食物中的多种食源性病原体都有效。因此,作为一种控制病原体的方法,纳米生物技术正在蓬勃发展。
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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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