Gold nanoparticles-based assays for biodetection in urine

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2021-08-01 DOI:10.1016/j.talanta.2021.122345

Maria António , Rui Vitorino , Ana L. Daniel-da-Silva

引用次数: 12

Abstract

Urine is a biofluid easy to collect through a non-invasive technique that allows collecting a large volume of sample. The use of urine for disease diagnosis is not yet well explored. However, it has gained attention over the last three years. It has been applied in the diagnosis of several illnesses such as kidney disease, bladder cancer, prostate cancer and cardiovascular diseases. In the last decade, gold nanoparticles (Au NPs) have attracted attention in biosensors' development for the diagnosis of diseases due to their electrical and optical properties, ability to conjugate with biomolecules, high sensitivity, and selectivity. Therefore, this article aims to present a comprehensive view of state of the art on the advances made in the quantification of analytes in urinary samples using AuNPs based assays, with a focus on protein analysis. The type of diagnosis methods, the Au NPs synthesis approaches and the strategies for surface modification aiming at selectivity towards the different targets are highlighted.

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基于金纳米颗粒的尿液生物检测方法

尿液是一种生物液体，易于通过非侵入性技术收集，可以收集大量样本。尿液在疾病诊断中的应用尚未得到很好的探索。然而，在过去的三年里，它引起了人们的关注。它已被应用于多种疾病的诊断，如肾病、膀胱癌、前列腺癌和心血管疾病。近十年来，金纳米颗粒(Au NPs)因其电学和光学特性、与生物分子的结合能力、高灵敏度和选择性等特点，在生物传感器诊断领域受到了广泛的关注。因此，本文旨在全面介绍使用基于AuNPs的测定法对尿液样本中分析物进行定量分析的最新进展，重点是蛋白质分析。重点介绍了诊断方法的类型、Au NPs的合成方法以及针对不同靶点的选择性表面修饰策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.