Chemical derivatization in LC-MS-based metabolomics study

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2020-10-01 DOI:10.1016/j.trac.2020.115988

Shuang Zhao, Liang Li

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引用次数: 77

Abstract

Because of great diversity of chemical and physical properties and wide ranges of concentrations of different metabolites in metabolome samples, metabolome analysis is a challenging task. Compromises in coverage, quantification accuracy and sample throughput are often used to provide a sufficient amount of metabolomic information for a biological or clinical application. Chemical derivatization of metabolites offers an opportunity to improve the overall analytical performance of liquid chromatography mass spectrometry (LC-MS)-based metabolomics. This review highlights recent progresses (since 2016) in the field of chemical derivatization LC-MS for both targeted and untargeted metabolome analysis. Due to increased recognition of the benefits of derivatization in LC-MS metabolome analysis, many research groups have been involved in advancing this active research field. Due to space limitation, we selected a few examples of recently reported derivatization methods and applications to showcase the advantages of chemical derivatization, compared to conventional label-free methods.

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基于lc - ms的代谢组学研究中的化学衍生化

由于代谢组样品中不同代谢物的化学和物理性质具有很大的多样性和浓度范围，因此代谢组分析是一项具有挑战性的任务。为了提供生物学或临床应用所需的足够量的代谢组学信息，通常会在覆盖范围、定量准确性和样品通量方面做出妥协。代谢物的化学衍生化为提高基于液相色谱-质谱(LC-MS)的代谢组学的整体分析性能提供了机会。本文综述了化学衍生LC-MS用于靶向和非靶向代谢组分析领域的最新进展(自2016年以来)。由于人们越来越认识到衍生化在LC-MS代谢组分析中的好处，许多研究小组已经参与推进这一活跃的研究领域。由于篇幅限制，我们选择了一些最近报道的衍生化方法和应用的例子来展示化学衍生化与传统无标签方法相比的优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.