{"title":"Angiogenesis <i>In Vitro</i> Utilizing Murine Vascular Explants in Miniaturized 3-Dimensional Collagen Gels.","authors":"May J Reed, Mamatha Damodarasamy, Robert B Vernon","doi":"10.2174/1877382601104010012","DOIUrl":null,"url":null,"abstract":"<p><p>Models of angiogenesis <i>in vitro</i> are used to study blood vessel morphogenesis and the effects of compounds that influence vascular growth. Herein, we describe techniques to induce angiogenesis-like sprouting from explants of mouse aortae and microvessels cultured in 3-dimensional gels of native type I collagen. The gels are supported by rings of nylon mesh that are sized to fit in 96-well culture plates. This mechanically-supported, miniaturized, 3-dimensional culture system requires only small quantities of cells and reagents and facilitates handling, staining, and imaging by conventional and confocal microscopy.</p>","PeriodicalId":90364,"journal":{"name":"The open circulation & vascular journal","volume":"4 ","pages":"12-17"},"PeriodicalIF":0.0000,"publicationDate":"2011-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972018/pdf/nihms519570.pdf","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The open circulation & vascular journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1877382601104010012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Models of angiogenesis in vitro are used to study blood vessel morphogenesis and the effects of compounds that influence vascular growth. Herein, we describe techniques to induce angiogenesis-like sprouting from explants of mouse aortae and microvessels cultured in 3-dimensional gels of native type I collagen. The gels are supported by rings of nylon mesh that are sized to fit in 96-well culture plates. This mechanically-supported, miniaturized, 3-dimensional culture system requires only small quantities of cells and reagents and facilitates handling, staining, and imaging by conventional and confocal microscopy.
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