Effect of bFGF on HLA-DR expression of human bone marrow-derived mesenchymal stem cells.

Q4 Biochemistry, Genetics and Molecular Biology
Journal of Stem Cells Pub Date : 2013-01-01
Pratiksha A Dighe, Pachaiyappan Viswanathan, Ashwin K Mruthunjaya, Raviraja N Seetharam
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Abstract

There has been a steady rise in the therapeutic applications of bone marrow mesenchymal stem cells (BM-MSCs) because of their unique properties of multilineage differentiation and immune modulation as well as the ease in isolation. However, up-regulation of surface HLA-DR levels when maintaining MSCs in culture under the influence of mitotic factors such as Basic fibroblast growth factor (bFGF) is an area of concern when considering them for the purpose of clinical applications. Thus, we investigated the association of bFGF supplemented to the culture media and the surface expression levels of HLA-DR in BM-MSCs in order to optimize the yield, while keeping HLA-DR levels under permissible levels. Human BM-MSCs were culture expanded in the absence of bFGF and in the presence of 1 ng/ml or 2 ng/ml bFGF. The HLA-DR profile of the cultures was analyzed at the end of each passage. On comparing the percent HLA-DR+ cell population at different concentrations as well as absence of bFGF, significant differences were not observed in the HLA-DR expression levels of the MSC cultures which had reached complete confluence. However, variations in HLA-DR expressions levels were seen which could be traced to the age of cells in culture with values drastically reduced to below 4% on maintaining MSCs typically two to three days beyond achieving full confluence. On the basis of the findings from this study, no significant correlation could be established on the effect of bFGF in modulating HLA-DR surface expression of BM-MSCs. Instead, the data are suggestive of the reasoning that the duration for which BM-MSCs are maintained in culture directly influences their phenotypic characteristics in terms of HLA-DR expression levels, with lowest levels achieved on their prolonged maintenance in culture.

bFGF对人骨髓间充质干细胞HLA-DR表达的影响。
骨髓间充质干细胞(BM-MSCs)由于其多系分化和免疫调节的独特特性以及易于分离,在治疗应用方面稳步上升。然而,在有丝分裂因子(如碱性成纤维细胞生长因子(bFGF))的影响下维持培养的MSCs时,表面HLA-DR水平的上调是考虑其临床应用时需要关注的一个领域。因此,我们研究了培养基中添加bFGF与BM-MSCs中HLA-DR表面表达水平的关系,以优化产量,同时将HLA-DR水平保持在允许的水平。在不含bFGF和存在1 ng/ml或2 ng/ml bFGF的情况下培养人BM-MSCs。在每个传代结束时分析培养物的HLA-DR谱。在比较不同浓度下HLA-DR+细胞群百分比以及不含bFGF的情况下,完全融合的MSC培养中HLA-DR表达水平无显著差异。然而,观察到HLA-DR表达水平的变化,这可以追溯到培养细胞的年龄,在维持MSCs通常超过完全融合两到三天的情况下,其值急剧降低至4%以下。基于本研究结果,bFGF对BM-MSCs HLA-DR表面表达的调节作用没有明显的相关性。相反,这些数据提示了这样一种推理,即BM-MSCs在培养中维持的时间直接影响其HLA-DR表达水平的表型特征,在培养中维持的时间越长,HLA-DR表达水平越低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Stem Cells
Journal of Stem Cells Medicine-Transplantation
CiteScore
0.10
自引率
0.00%
发文量
1
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