Fubin Pei , Shasha Feng , Yuehua Zhang , Yi Wu , Chenglong Chen , Yue Sun , Zihao Xie , Qingli Hao , Yang Cao , Zhaoyang Tong , Wu Lei
{"title":"A photoelectrochemical immunosensor based on Z-scheme CdS composite heterojunction for aflatoxin B1","authors":"Fubin Pei , Shasha Feng , Yuehua Zhang , Yi Wu , Chenglong Chen , Yue Sun , Zihao Xie , Qingli Hao , Yang Cao , Zhaoyang Tong , Wu Lei","doi":"10.1016/j.bios.2022.114500","DOIUrl":null,"url":null,"abstract":"<div><p><span>Aflatoxin B1<span> (AFB1) is a highly toxic fungal contaminant widely found in agricultural products. It causes serious harm to human health and the environment. Thus, a fast and sensitive detection<span> approach is urgently needed to prevent AFB1-contaminated products from entering the market effectively. A photoelectrochemical (PEC) immunosensor was developed based on tungsten<span> trioxide/cadmium sulfide core/shell coated with a composite layer consisting of polydopamine and loaded gold nanoparticles (WO</span></span></span></span><sub>3</sub><span>/CdS@PDA/Au) for AFB1 detection. CdS formed a heterojunction with WO</span><sub>3</sub><span>, which improved the photoelectric performance. The coated PDA reducing CdS toxicity was demonstrated by biological experiment of </span><span><em>Bacillus subtilis</em></span><span>. PDA and Au NPs promoted electron transfer<span> between the semiconductors, being beneficial promoting the photoelectron transfer. Additionally, the antibodies were immobilized on WO</span></span><sub>3</sub><span>/CdS@PDA/Au via the reactive quinones on the surface of the PDA and electrostatic adsorption from Au NPs. The WO</span><sub>3</sub><span><span>/CdS@PDA/Au composite as a Z-scheme heterojunction possessed high performance of photocurrent response, and the photoproduced electron/hole transfer path was speculated by electrons spin-resonance spectroscopy technique. Under the optimum experimental conditions, the PEC immunosensor showed a wide </span>linear detection range from 0.05 to 100 ng mL</span><sup>−1</sup> for AFB1, indicating that the immunosensor has a bright application prospect.</p></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":null,"pages":null},"PeriodicalIF":10.7000,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566322005401","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 17
Abstract
Aflatoxin B1 (AFB1) is a highly toxic fungal contaminant widely found in agricultural products. It causes serious harm to human health and the environment. Thus, a fast and sensitive detection approach is urgently needed to prevent AFB1-contaminated products from entering the market effectively. A photoelectrochemical (PEC) immunosensor was developed based on tungsten trioxide/cadmium sulfide core/shell coated with a composite layer consisting of polydopamine and loaded gold nanoparticles (WO3/CdS@PDA/Au) for AFB1 detection. CdS formed a heterojunction with WO3, which improved the photoelectric performance. The coated PDA reducing CdS toxicity was demonstrated by biological experiment of Bacillus subtilis. PDA and Au NPs promoted electron transfer between the semiconductors, being beneficial promoting the photoelectron transfer. Additionally, the antibodies were immobilized on WO3/CdS@PDA/Au via the reactive quinones on the surface of the PDA and electrostatic adsorption from Au NPs. The WO3/CdS@PDA/Au composite as a Z-scheme heterojunction possessed high performance of photocurrent response, and the photoproduced electron/hole transfer path was speculated by electrons spin-resonance spectroscopy technique. Under the optimum experimental conditions, the PEC immunosensor showed a wide linear detection range from 0.05 to 100 ng mL−1 for AFB1, indicating that the immunosensor has a bright application prospect.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.