In vitro osteogenic potential of canine adipose derived stem cells.

Q4 Biochemistry, Genetics and Molecular Biology
Mansi K Aparnathi, Jasminkumari A Patel, Ajai K Tripathi, Devshibhai R Barvalia, Chaitanya G Joshi
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引用次数: 0

Abstract

Stem cells based tissue engineering is a promising approach for the regenerative treatment of various tissue disorders. Adipose tissue is an abundant source of cells which are competent of multipotential differentiation, called adipose-derived stem cells (ADSCs). The present study was contemplated with the objective of assessing the osteogenic differentiation potential of the canine ADSCs in vitro. The canine ADSCs were isolated from adipose tissue around falciform ligament and abdominal subcutaneous fat. Yield of viable ADSCs from both the tissue sources was found to be nearly equivalent. Tissue subjected to trypsinization yielded more viable, but lesser number of cells as compared to collagenase treatment. The stemness of ADSCs was affirmed by reverse transcriptase PCR which exhibited the expression of stem cell specific genes, OCT4 and NANOG.The monolayer of ADSCs was subjected to differentiation into adipogenic and osteogenic lineages. Assessment of the osteogenic potential of ADSCs in vitro opens a new therapeutic horizon for development of in vivo strategies employing autologous stem cell based tissue regeneration in orthopedics.

犬脂肪干细胞体外成骨潜能的研究。
基于干细胞的组织工程是一种很有前途的再生治疗各种组织疾病的方法。脂肪组织是具有多能分化能力的细胞的丰富来源,称为脂肪来源干细胞(ADSCs)。本研究旨在评估犬ADSCs在体外的成骨分化潜力。从镰状韧带周围脂肪组织和腹部皮下脂肪中分离犬ADSCs。两种组织来源的可活ADSCs的产量几乎相等。与胶原酶处理相比,胰蛋白酶化组织产生了更多的活细胞,但细胞数量较少。逆转录酶PCR证实了ADSCs的干性,显示了干细胞特异性基因OCT4和NANOG的表达。单层ADSCs分化为成脂系和成骨系。体外对ADSCs成骨潜能的评估,为骨科中基于自体干细胞的组织再生的体内策略的发展开辟了新的治疗前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Stem Cells
Journal of Stem Cells Medicine-Transplantation
CiteScore
0.10
自引率
0.00%
发文量
1
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