Circadian behavior of mice deficient in PER1/PML or PER2/PML.

Q2 Biochemistry, Genetics and Molecular Biology

Journal of Circadian Rhythms Pub Date : 2013-08-28 DOI:10.1186/1740-3391-11-9

Takao Miki, Misty Chen-Goodspeed, Zhaoyang Zhao, Cheng Chi Lee

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引用次数: 5

Abstract

Background: Our recent studies demonstrate that the murine homolog of the human tumor suppressor promyelocytic leukemia (PML) regulates circadian behavior of mice. To further gather insight into PML's contribution to circadian behavior, we generated two strains of mice deficient in one of the two period (Per) genes and the PML gene, with Per1-/-/Pml-/- and Per2-/-/Pml-/- genotypes.

Results: Here we report the circadian behavior of these mice based on wheel-running behavioral analysis. In a free-running environment, the Per1-/-/Pml-/- mice maintained circadian rhythm but displayed a significantly shorter period of 22.2 h. In addition, these mice displayed significantly enhanced phase response to a light pulse given at zeitgeber time (ZT) 14 and 22. The Per2-/-/Pml-/- mice lose persistent rhythm when in a free-running environment, as also the case for Per2-/- mice. A transient post-light pulse rhythm seen in the arrhythmic Per2-/- mice was less apparent in Per2-/-/Pml-/- mice. Both the Per1-/-/Pml-/- and Per2-/-/Pml-/- mice displayed a more advanced phase angle of entrainment activity during light-dark cycles than the single gene deficient mice.

Conclusions: Beyond merely regulating PER1 and PER2, the current behavioral studies suggest PML has additional roles in mouse circadian behavior.

Abstract Image

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PER1/PML或PER2/PML缺失小鼠的昼夜行为

背景:我们最近的研究表明，人类肿瘤抑制因子早幼粒细胞白血病(PML)的小鼠同源物调节小鼠的昼夜节律行为。为了进一步了解PML对昼夜节律行为的贡献，我们培养了两株缺乏两个周期(Per)基因之一和PML基因的小鼠，分别为Per1-/-/ PML -/-和Per2-/-/ PML -/-基因型。结果:在此，我们报告了这些小鼠基于轮跑行为分析的昼夜节律行为。在自由运动环境下，Per1-/-/Pml-/-小鼠维持了昼夜节律，但其周期明显缩短，为22.2 h。此外，这些小鼠对授时时间(ZT) 14和22的光脉冲表现出显著增强的相位响应。Per2-/-/Pml-/-小鼠在自由奔跑的环境中失去了持久的节律，Per2-/-小鼠也是如此。在Per2-/-/Pml-/-小鼠中看到的瞬时光后脉冲节律在Per2-/-/Pml-/-小鼠中不太明显。与单基因缺陷小鼠相比，Per1-/-/Pml-/-和Per2-/-/ /Pml-/-小鼠在光-暗循环中表现出更先进的夹带活动相角。结论:除了仅仅调节PER1和PER2外，目前的行为研究表明PML在小鼠昼夜节律行为中还有其他作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Circadian Rhythms Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

7.10

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Journal of Circadian Rhythms is an Open Access, peer-reviewed online journal that publishes research articles dealing with circadian and nycthemeral (daily) rhythms in living organisms, including processes associated with photoperiodism and daily torpor. Journal of Circadian Rhythms aims to include both basic and applied research at any level of biological organization (molecular, cellular, organic, organismal, and populational). Studies of daily rhythms in environmental factors that directly affect circadian rhythms are also pertinent to the journal"s mission.