Determination of aflatoxin m1 in milk by ELISA technique in mashad (northeast of iran).

ISRN Toxicology Pub Date : 2012-08-23 Print Date: 2012-01-01 DOI:10.5402/2012/121926
Ali Mohamadi Sani, Mohamad Khezri, Halime Moradnia
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引用次数: 29

Abstract

The aim of this study was to detect the amount of aflatoxin M1 (AFM1) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 97.6% of the examined milk samples by average concentration of 23 ± 16 ppt and contamination level ranging between 6 and 71 ppt. The concentration of AFM1 in all the samples was lower than the Iranian national standard and Food and Drug Administration limits (500 ppt), and, only in 3 (1.6%) samples, AFM1 concentration was more than the maximum tolerance limit (50 ppt) accepted by European Union and Codex Alimentarius Commission. According to our findings and previous studies, AFM1 contamination of milk is not a concern in this region, and the regional standard of AFM1 contamination in milk might be changed to lower than 100 ppt.

Abstract Image

ELISA法测定伊朗东北部马什哈德地区牛奶中的黄曲霉毒素m1。
本研究的目的是检测伊朗东北部马什哈德巴氏消毒牛奶样品中黄曲霉毒素M1 (AFM1)的含量。为此,研究人员于2011年秋季从零售商店收集了42份牛奶样品,并采用酶联免疫吸附试验(ELISA)技术对AFM1进行了分析。所有的分析都做了两次。结果显示,97.6%的牛奶样品中存在AFM1,平均浓度为23±16 ppt,污染水平在6 ~ 71 ppt之间。所有样品中AFM1浓度均低于伊朗国家标准和食品药品监督管理局限量(500 ppt),仅有3份(1.6%)样品AFM1浓度超过欧盟和食品法典委员会认可的最大容许限量(50 ppt)。根据我们的发现和之前的研究,该地区牛奶中AFM1的污染不是一个问题,牛奶中AFM1污染的地区标准可能会更改为低于100 ppt。
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