A portable smartphone-assisted highly emissive magnetic covalent organic framework-based fluorescence sensor for the detection of Salmonella typhimurium
Shengnan Wei , Feng Wang , Liang Zhang , Chao Zhao , Juan Li , Juan Wang
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引用次数: 1
Abstract
The core/shell magnetic covalent organic framework doped with gold nanoparticles (MCOF-AuNPs) was prepared by a facile two-step in situ synthesis strategy to achieve high emission. The fluorescence emission was enhanced and overcame the aggregation-induced quenching of COF after the introduction of Fe3O4 and AuNPs. Interestingly, the emission can be quenched by Fe3+ ions. MCOF-AuNPs combined with a specific aptamer (apt-MCOF-AuNPs) can capture Salmonella typhimurium selectively, and Fe3+-induced fluorescence quenching was inhibited based on the competitive binding of Fe3+ ions. A fluorescent assay strategy was constructed with excellent specificity and sensitivity. The detection limit is as low as 4 CFU/mL within 33 min. Furthermore, we developed smartphone-based software as an image reader and analyzer to determine S. typhimurium by capturing the change in fluorescence lightness, which can be assisted for point-of-care testing. Collectively, this innovative design showed a promising on-site detection approach for S. typhimurium.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.