Early degenerative effects of diabetes mellitus on pancreas, liver, and kidney in rats: an immunohistochemical study.

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-07-11 DOI:10.1155/2012/120645
Mehmet Haligur, Senay Topsakal, Ozlem Ozmen
{"title":"Early degenerative effects of diabetes mellitus on pancreas, liver, and kidney in rats: an immunohistochemical study.","authors":"Mehmet Haligur,&nbsp;Senay Topsakal,&nbsp;Ozlem Ozmen","doi":"10.1155/2012/120645","DOIUrl":null,"url":null,"abstract":"<p><p>Liver and kidney commonly affected by diabetes in chronic cases but pathogenetic mechanisms are not fully understood in early stages of the disease. The aim of this study was to investigate the immunohistochemical expression of caspase-3, cyclooxygenase (COX)-1 and-2, calcium sensing receptor (CSR), and hypoxia inducible factor-1α (HIF-1α) in pancreas, liver, and kidney in streptozotocin (STZ) induced DM. Study group (n = 6) were received streptozotocin (50 mg/kg) and control group (n = 6) physiologic saline. The blood glucose and ketonuria were measured, and necropsy was performed on them on third, fourth, and fifth days. Immunohistochemistry revealed that marked increase in caspase-3 reaction pancreas, liver, and kidney in the study group than control group. COX-1 slightly increased in these organs in study group compared to controls. Immunohistochemically COX-2 reaction was markedly positive in liver and kidney, but slightly increased in pancreas. The most increased reaction was observed in CRS and all organs were markedly positive. HIF-1α expression was also increased but the reaction was more severe in pancreas than liver and kidney. This study indicated that degeneration starts in organs in early stages of the disease and the most effective route for degeneration related to increase of calcium influx and hypoxia upon cells in DM.</p>","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/120645","citationCount":"41","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Diabetes Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2012/120645","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/7/11 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 41

Abstract

Liver and kidney commonly affected by diabetes in chronic cases but pathogenetic mechanisms are not fully understood in early stages of the disease. The aim of this study was to investigate the immunohistochemical expression of caspase-3, cyclooxygenase (COX)-1 and-2, calcium sensing receptor (CSR), and hypoxia inducible factor-1α (HIF-1α) in pancreas, liver, and kidney in streptozotocin (STZ) induced DM. Study group (n = 6) were received streptozotocin (50 mg/kg) and control group (n = 6) physiologic saline. The blood glucose and ketonuria were measured, and necropsy was performed on them on third, fourth, and fifth days. Immunohistochemistry revealed that marked increase in caspase-3 reaction pancreas, liver, and kidney in the study group than control group. COX-1 slightly increased in these organs in study group compared to controls. Immunohistochemically COX-2 reaction was markedly positive in liver and kidney, but slightly increased in pancreas. The most increased reaction was observed in CRS and all organs were markedly positive. HIF-1α expression was also increased but the reaction was more severe in pancreas than liver and kidney. This study indicated that degeneration starts in organs in early stages of the disease and the most effective route for degeneration related to increase of calcium influx and hypoxia upon cells in DM.

Abstract Image

Abstract Image

Abstract Image

糖尿病对大鼠胰腺、肝脏和肾脏的早期退行性影响:免疫组织化学研究。
肝脏和肾脏通常受慢性糖尿病的影响,但在疾病的早期发病机制尚不完全清楚。本研究旨在探讨链脲佐菌素(STZ)诱导DM大鼠胰腺、肝脏和肾脏组织中caspase-3、环氧化酶(COX)-1和-2、钙敏感受体(CSR)和缺氧诱导因子-1α (HIF-1α)的免疫组化表达。研究组(n = 6)给予链脲佐菌素(50 mg/kg),对照组(n = 6)给予生理盐水。测定血糖和尿酮量,于第3、4、5天进行尸检。免疫组化显示,研究组胰腺、肝脏、肾脏的caspase-3反应明显高于对照组。与对照组相比,研究组这些器官中的COX-1略有升高。免疫组织化学COX-2反应在肝脏和肾脏中明显阳性,在胰腺中略有升高。CRS反应增加最多,各脏器均明显阳性。HIF-1α在胰腺中的表达明显高于肝脏和肾脏。本研究表明,变性始于疾病早期的器官,最有效的变性途径与糖尿病细胞钙内流增加和缺氧有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Experimental Diabetes Research
Experimental Diabetes Research 医学-内分泌学与代谢
自引率
0.00%
发文量
0
审稿时长
3-8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信