Extraction optimization and structural characterization of soluble dietary fiber in foxtail millet and its inhibitory activities on colon cancer

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2023-08-01 DOI:10.1016/j.jff.2023.105659

Aiqi Ren , Liying Chen , Wenjing Zhao , Shuhua Shan , Zhuoyu Li , Zhaohui Tang

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引用次数: 1

Abstract

Soluble dietary fiber has anti-tumor effect and is closely related to human health. At present the structure of soluble dietary fiber from foxtail millet (FMB-SDF) and its anti-tumor effect remain unclear. In this study, the structure of FMB-SDF was characterized, and its effects on the colon cancer were investigated. The results showed that under optimal extraction conditions the yield of FMB-SDF was 14.25 %. Based on the monosaccharide composition, methylation results and NMR analysis, the preliminary structure of FMB-SDF was inferred to be a glucan. Interestingly, FMB-SDF markedly inhibited the colony formation ability of HCT116 and HT-29 cells and could significantly induce the increase of reactive oxygen species (ROS) and apoptosis of HCT116 and HT-29 cells. The results of animal experiments showed that FMB-SDF could significantly alleviate clinical symptoms of colon cancer in mice. These results provide a theoretical basis for the exploitation and utilization of FMB-SDF against colon cancer.

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谷子可溶性膳食纤维的提取优化、结构表征及其对结肠癌的抑制作用

可溶性膳食纤维具有抗肿瘤作用，与人体健康密切相关。目前对谷子可溶性膳食纤维(FMB-SDF)的结构及其抗肿瘤作用尚不清楚。本研究对FMB-SDF的结构进行了表征，并对其在结肠癌中的作用进行了探讨。结果表明，在最佳提取条件下，FMB-SDF得率为14.25%。根据单糖组成、甲基化结果和核磁共振分析，推测FMB-SDF的初步结构为葡聚糖。有趣的是，FMB-SDF显著抑制HCT116和HT-29细胞的集落形成能力，并能显著诱导HCT116和HT-29细胞活性氧(ROS)的增加和凋亡。动物实验结果显示，FMB-SDF能显著缓解小鼠结肠癌的临床症状。这些结果为FMB-SDF抗结肠癌的开发利用提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.

文献相关原料

公司名称	产品信息	采购帮参考价格
上海源叶	cellulose