N-acetylcysteine ameliorates nitrosative stress on radiation-inducible damage in rat liver.

Q2 Medicine

Journal of Buon Pub Date : 2011-01-01

S Kilciksiz, C Demirel, S Evirgen Ayhan, N Erdal, S Gurgul, L Tamer, L Ayaz

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引用次数: 0

Abstract

Purpose: The present study was designed to investigate the potential radioprotective effects of N-acetylcysteine (NAC) on radiation-induced nitrosative stress caused by gamma irradiation (single dose, 6 Gy) in rat liver.

Methods: The rats (n=40) were divided randomly and equally into 4 groups: Control (C), Radiation (R), R+NAC (received irradiation and 1,000 mg/kg of NAC) and R+WR-2721 (received irradiation and 200 mg/kg of WR-2721). Liver tissue of each animal was harvested and utilized for 3-nitrotyrosine (3-NT) detection using high-performance liquid chromatography- ultraviolet (HPLC-UV) system.

Results: In the R rats, 3-NT levels significantly increased when compared to those of the C rats (p<0.05). There were no significant differences in the 3-NT levels among R+NAC and R+WR-2721 rats. Histologically examined liver tissue samples showed no obvious differences.

Conclusion: The present study suggests that irradiation has a negative effect on the cellular proteins by enhancing 3-NT formation. The prophylactic use of NAC seems to reduce the nitrosative damage during radiotherapy.

本刊更多论文

n-乙酰半胱氨酸改善亚硝化应激对辐射诱导大鼠肝脏损伤的影响。

目的:研究n-乙酰半胱氨酸(NAC)对γ辐照(单剂量，6 Gy)所致大鼠肝脏亚硝化应激的潜在防护作用。方法:将40只大鼠随机等分分为4组:对照组(C)、辐射组(R)、R+NAC组(照射组，NAC剂量为1000 mg/kg)和R+WR-2721组(照射组，WR-2721剂量为200 mg/kg)。取肝组织，利用高效液相色谱-紫外(HPLC-UV)系统检测3-硝基酪氨酸(3-NT)。结果:与C大鼠相比，R大鼠3-NT水平明显升高(p)。结论:本研究提示辐照通过促进3-NT的形成而对细胞蛋白产生负面影响。预防性使用NAC似乎可以减少放射治疗期间的亚硝化损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Buon 医学-肿瘤学

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： JBUON aims at the rapid diffusion of scientific knowledge in Oncology. Its character is multidisciplinary, therefore all aspects of oncologic activities are welcome including clinical research (medical oncology, radiation oncology, surgical oncology, nursing oncology, psycho-oncology, supportive care), as well as clinically-oriented basic and laboratory research, cancer epidemiology and social and ethical aspects of cancer. Experts of all these disciplines are included in the Editorial Board. With a rapidly increasing body of new discoveries in clinical therapeutics, the molecular mechanisms that contribute to carcinogenesis, advancements in accurate and early diagnosis etc, JBUON offers a free forum for clinicians and basic researchers to make known promptly their achievements around the world. With this aim JBUON accepts a broad spectrum of articles such as editorials, original articles, reviews, special articles, short communications, commentaries, letters to the editor and correspondence among authors and readers. JBUON keeps the characteristics of its former paper print edition and appears as a bimonthly e-published journal with continuous volume, issue and page numbers.