Angiotensin II modifies the expression of α1-adrenoceptors in aorta smooth muscle cells of α1D-adrenoceptor knockout mice

M. L. Lázaro-Suárez, J. H. Gómez-Zamudio, N. L. Delgado-Buenrostro, A. Tanoue, G. Tsujimoto, R. Villalobos-Molina
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引用次数: 2

Abstract

1 The effect of angiotensin II (Ang II) on α1A-, α1B-, and a1D-adrenoceptors (α1-AR) expression was analyzed in aorta smooth muscle cells obtained from wild-type (WT) and knock out of α1D-AR (α1D-AR KO) mice.

2 The relative abundance of mRNA for the three α1-ARs was determined in WT and α1D-AR KO aortic smooth muscle cells. There were no significant differences between WT and α1D-AR KO cells.

3 As early as 1 h Ang II increased α1B-AR mRNA in WT cells ≈ 2 fold compared with control; in contrast, in α1D-AR KO cells the α1B-AR transcript was ≈ 50% of control.

4 Western blot assays showed that Ang II incremented protein content for α1A-AR, 86% and 107% in WT and α1D-AR KO cells, respectively.

5 Protein for α1B- and α1D-ARs did not change significantly with Ang II in both WT and a1D-AR KO cells.

6 The effect of Ang II on α1B-AR mRNA seems to be influenced by the absence of α1D-AR in aortic smooth muscle cells, which might be important to understand the interactions among α1-ARs.

血管紧张素II改变α 1d -肾上腺素受体敲除小鼠主动脉平滑肌细胞中α1-肾上腺素受体的表达
1 .分析血管紧张素II (Ang II)对α1D-AR (α1D-AR KO)野生型和敲除型小鼠主动脉平滑肌细胞α1A-、α1B-和α 1d -肾上腺素受体(α1-AR)表达的影响。2测定WT和α1D-AR KO主动脉平滑肌细胞中3种α1- ar mRNA的相对丰度。WT与α1D-AR KO细胞间差异无统计学意义。3早在1 h时,Angⅱ使WT细胞α1B-AR mRNA表达量较对照增加约2倍;而在α1D-AR KO细胞中,α1B-AR转录物约为对照组的50%。4 . Western blot检测显示,angii使WT和α1D-AR - KO细胞中α1A-AR蛋白含量分别增加了86%和107%。5在WT和a1D-AR KO细胞中α1B-和α1D-ARs蛋白均未随Ang II发生显著变化。6 angii对α1B-AR mRNA的影响可能受到主动脉平滑肌细胞中α1D-AR缺失的影响,这可能对了解α1- ar之间的相互作用具有重要意义。
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