Evaluation of peroxidase activity by alpha-naphthol/pyronine staining compared with benzidine staining in 101 acute leukemia cases.

Véronique Latger-Cannard, Valérie Bardet, Michèle Malet, Monique Lagrange, Fabienne Empereur, Odile Fenneteau
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引用次数: 3

Abstract

Cytochemical detection of myeloperoxidase (MPO) activity, a strong marker for myeloid differentiation, is usually performed by benzidine dihydrochloride staining, with the threshold at 3%. Several reports have demonstrated the potential toxicity of benzidine, and bans have been issued, under French law, prohibiting female technicians from being exposed to the aromatic hydrocarbon group, including benzidine. The aim of this study was to test an alpha-naphthol and pyronine-based substitute using a standardized kit (MYELOPEROXIDASE KIT, RAL [Réactifs RAL, Martillac, France]) to measure MPO activity in blast cells. This prospective, multicenter study made it possible to analyze 101 acute leukemia (AL) cases; it has also demonstrated both the 96% specificity and the 99% sensitivity of the method, with a threshold for positive staining of 3%, as well as good correlation (r = 0.95) between the staining method tested and the benzidine staining method. When using the alpha-naphthol/pyronine-based staining for MPO, the mean number of positive blast cells is statistically lower than that obtained using benzidine, but without incidence on AL classification. These results allow us to conclude that this method makes it possible to classify acute blood diseases by measuring MPO activity using reagents permitted by law, according to a standardized and reproducible protocol.
101例急性白血病患者用-萘酚/吡啶染色与联苯胺染色比较过氧化物酶活性。
髓过氧化物酶(MPO)活性的细胞化学检测是髓细胞分化的重要标志,通常采用盐酸联苯胺染色,阈值为3%。几份报告已经证明了联苯胺的潜在毒性,根据法国法律,已经颁布禁令,禁止女性技术人员接触包括联苯胺在内的芳烃基团。本研究的目的是使用标准化试剂盒(髓过氧化物酶试剂盒,RAL [r actifs RAL, Martillac,法国])测试α -萘酚和吡啶基替代品,以测量母细胞中的MPO活性。这项前瞻性、多中心研究使101例急性白血病(AL)病例分析成为可能;结果表明,该方法特异性为96%,灵敏度为99%,阳性染色阈值为3%,所检测的染色方法与联苯胺染色方法具有良好的相关性(r = 0.95)。采用α -萘酚/吡啶染色法检测MPO时,阳性母细胞的平均数量在统计学上低于联苯胺染色法,但与AL分类无关。这些结果使我们得出结论,该方法可以根据标准化和可重复的协议,通过使用法律允许的试剂测量MPO活性来对急性血液病进行分类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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