Rapid construction of adeno-associated virus vectors expressing multiple short hairpin RNAs with high antiviral activity against echovirus 30.

Diana Rothe, Gisela Wajant, Hans-Peter Grunert, Heinz Zeichhardt, Henry Fechner, Jens Kurreck
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引用次数: 13

Abstract

RNA interference has proven to be a powerful tool to inhibit viruses. For the prevention of viral escape, multiple short hairpin RNAs (shRNAs) will have to be employed. This article describes a rapid procedure for the generation of shRNA expression cassettes by parallel cloning as well as a simple strategy for the combination of selected units. After delivery of the shRNA expression cassettes with adeno-associated virus vectors, inhibition of echovirus 30 as well as silencing of an important cellular cofactor of virus replication were achieved. The procedure has the potential to be generally applicable for silencing of multiple endogenous targets or viruses.

表达多个具有高抗病毒活性的短发夹rna的腺相关病毒载体的快速构建。
RNA干扰已被证明是抑制病毒的有力工具。为了防止病毒逃逸,必须使用多个短发夹rna (shrna)。本文描述了一种通过平行克隆快速生成shRNA表达盒的方法,以及一种选择单位组合的简单策略。在用腺相关病毒载体递送shRNA表达盒后,实现了对echovirus 30的抑制以及对病毒复制的重要细胞辅助因子的沉默。该程序有可能普遍适用于多种内源性靶标或病毒的沉默。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Oligonucleotides
Oligonucleotides 生物-生化与分子生物学
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