A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification.

Q4 Medicine

Archives of histology and cytology Pub Date : 2009-01-01 DOI:10.1679/aohc.72.175

Kanae Kumagishi, Keiichiro Nishida, Tomoichiro Yamaai, Ryusuke Momota, Shigeru Miyaki, Satoshi Hirohata, Ichiro Naito, Hiroshi Asahara, Yoshifumi Ninomiya, Aiji Ohtsuka

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引用次数: 7

Abstract

A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) is known to influence aggrecan degradation in endochondral ossification, but its role has not been well understood. In the present study, in vitro gene expression of ADAMTS9 was investigated by RT-PCR in ATDC5 cells in which experimentally chondrogenic differentiation had been induced. We also investigated the protein localization and gene expression pattern of ADAMTS9 in the tibia growth plate cartilage of male mice in a day 1 neonate, 7-week-old young adult, and a 12-week-old adult by immunohistochemistry and in situ hybridization and compared the results with the expression of proliferating cell nuclear antigen (PCNA) and type X collagen for the identification of proliferative and hypertrophic chondrocyte phenotypes, respectively. We found the gene expression of ADAMTS9 by ATDC5 cells as a dual mode, both before the expression of type X collagen and after hypertrophic differentiation. The immunoreactivity of ADAMTS9 was observed in chondrocytes of proliferative and hypertrophic zones in the growth plate. The population of ADAMTS9 positive cells decreased with age. The results of the present study suggest that ADAMTS9 might have a role in aggrecan cleavage around the chondrocytes to allow chondrocyte proliferation and hypertrophy.

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软骨细胞在软骨内成骨过程中表达的具有血小板反应蛋白基序9 (ADAMTS9)的崩解素和金属蛋白酶。

已知具有血栓反应蛋白基序的崩解素和金属蛋白酶9 (ADAMTS9)在软骨内成骨过程中影响聚集蛋白降解，但其作用尚未得到很好的理解。在本研究中，采用RT-PCR方法研究了ADAMTS9基因在实验性诱导成软骨分化的ATDC5细胞中的体外表达。我们还通过免疫组化和原位杂交的方法研究了ADAMTS9在1日龄新生、7周龄青年和12周龄成年雄性小鼠胫骨生长板软骨中的蛋白定位和基因表达模式，并将结果与增殖细胞核抗原(PCNA)和X型胶原蛋白的表达进行了比较，分别用于鉴别增生性和增生性软骨细胞表型。我们发现ADAMTS9基因在ATDC5细胞中的表达为双重模式，即在X型胶原表达前和增生性分化后。在生长板增殖带和肥大带软骨细胞中观察到ADAMTS9的免疫反应性。ADAMTS9阳性细胞的数量随着年龄的增长而减少。本研究的结果表明，ADAMTS9可能在软骨细胞周围的聚集蛋白裂解中起作用，从而使软骨细胞增殖和肥大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of histology and cytology 生物-细胞生物学

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期刊介绍： The Archives of Histology and Cytology provides prompt publication in English of original works on the histology and histochemistry of man and animals. The articles published are in principle restricted to studies on vertebrates, but investigations using invertebrates may be accepted when the intention and results present issues of common interest to vertebrate researchers. Pathological studies may also be accepted, if the observations and interpretations are deemed to contribute toward increasing knowledge of the normal features of the cells or tissues concerned. This journal will also publish reviews offering evaluations and critical interpretations of recent studies and theories.