Upregulation of hyaluronan and its binding receptors in an experimental model of chronic cyclosporine nephropathy.

Dong He Han, Hyun Kuk Song, So Young Lee, Ji-Hyun Song, Shang Guo Piao, Hye Eun Yoon, Jung Yeon Ghee, Hyung Ju Yoon, Jin Kim, Chul Woo Yang
{"title":"Upregulation of hyaluronan and its binding receptors in an experimental model of chronic cyclosporine nephropathy.","authors":"Dong He Han,&nbsp;Hyun Kuk Song,&nbsp;So Young Lee,&nbsp;Ji-Hyun Song,&nbsp;Shang Guo Piao,&nbsp;Hye Eun Yoon,&nbsp;Jung Yeon Ghee,&nbsp;Hyung Ju Yoon,&nbsp;Jin Kim,&nbsp;Chul Woo Yang","doi":"10.1111/j.1440-1797.2009.01167.x","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>Hyaluronan (HA) is an important extracellular matrix (ECM) proteoglycan. The localization of HA and its binding receptors, CD44 and LYVE-1, was evaluated in an experimental model of chronic cyclosporine A (CsA)-induced nephropathy.</p><p><strong>Methods: </strong>Sprague-Dawley rats maintained on a low-salt diet (0.05% sodium) received an s.c. injection of vehicle (1 mL/kg per day olive oil; VH groups) or CsA (15 mg/kg per day; CsA groups) for 1 or 4 weeks. Induction of chronic CsA nephropathy was evaluated according to renal function and pathology and expression of HA, CD44, LYVE-1, ED-1 and alpha-smooth muscle actin (alpha-SMA).</p><p><strong>Results: </strong>CsA treatment for 4 weeks caused renal dysfunction, which was accompanied by typical striped interstitial fibrosis. In the VHroup, HA immunoreactivity was observed only in the inner medulla. However, the area of HA immunoreactivity increased with the duration of CsA treatment: CsA treatment for 1 week extended HA immunoreactivity to the outer medulla, and CsA treatment for 4 weeks caused a further extension of HA immunoreactivity to the cortex, which was vulnerable to CsA-induced renal injury. HA binding receptor, CD44 and LYVE-1 expression were also upregulated in the CsA groups, and were localized to the area of fibrosis and the peritubular capillaries of the cortex. In the CsA groups, ED-1 and alpha-SMA were predominantly expressed in fibrotic areas in which HA had accumulated.</p><p><strong>Conclusion: </strong>These findings suggest that upregulation of HA and its binding receptors are involved in interstitial fibrosis in chronic CsA-induced renal injury.</p>","PeriodicalId":520716,"journal":{"name":"Nephrology (Carlton, Vic.)","volume":" ","pages":"216-24"},"PeriodicalIF":0.0000,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1797.2009.01167.x","citationCount":"18","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nephrology (Carlton, Vic.)","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/j.1440-1797.2009.01167.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18

Abstract

Aim: Hyaluronan (HA) is an important extracellular matrix (ECM) proteoglycan. The localization of HA and its binding receptors, CD44 and LYVE-1, was evaluated in an experimental model of chronic cyclosporine A (CsA)-induced nephropathy.

Methods: Sprague-Dawley rats maintained on a low-salt diet (0.05% sodium) received an s.c. injection of vehicle (1 mL/kg per day olive oil; VH groups) or CsA (15 mg/kg per day; CsA groups) for 1 or 4 weeks. Induction of chronic CsA nephropathy was evaluated according to renal function and pathology and expression of HA, CD44, LYVE-1, ED-1 and alpha-smooth muscle actin (alpha-SMA).

Results: CsA treatment for 4 weeks caused renal dysfunction, which was accompanied by typical striped interstitial fibrosis. In the VHroup, HA immunoreactivity was observed only in the inner medulla. However, the area of HA immunoreactivity increased with the duration of CsA treatment: CsA treatment for 1 week extended HA immunoreactivity to the outer medulla, and CsA treatment for 4 weeks caused a further extension of HA immunoreactivity to the cortex, which was vulnerable to CsA-induced renal injury. HA binding receptor, CD44 and LYVE-1 expression were also upregulated in the CsA groups, and were localized to the area of fibrosis and the peritubular capillaries of the cortex. In the CsA groups, ED-1 and alpha-SMA were predominantly expressed in fibrotic areas in which HA had accumulated.

Conclusion: These findings suggest that upregulation of HA and its binding receptors are involved in interstitial fibrosis in chronic CsA-induced renal injury.

慢性环孢素肾病实验模型中透明质酸及其结合受体的上调。
目的:透明质酸(HA)是重要的细胞外基质(ECM)蛋白多糖。在慢性环孢素A (CsA)肾病的实验模型中,评估HA及其结合受体CD44和LYVE-1的定位。方法:维持低盐饮食(0.05%钠)的Sprague-Dawley大鼠皮下注射1 mL/kg / d橄榄油;VH组)或CsA(每天15 mg/kg;CsA组)治疗1或4周。根据肾功能、病理及HA、CD44、LYVE-1、ED-1、α -平滑肌肌动蛋白(α - sma)的表达情况评估慢性CsA肾病的诱导程度。结果:CsA治疗4周后出现肾功能不全,伴有典型的条纹间质纤维化。在v组中,仅在内髓质中观察到HA免疫反应。然而,HA免疫反应的范围随着CsA治疗时间的延长而增加:CsA治疗1周使HA免疫反应延伸至外髓质,CsA治疗4周使HA免疫反应进一步延伸至皮质,皮质易发生CsA引起的肾损伤。HA结合受体、CD44和LYVE-1的表达在CsA组中也上调,并局限于纤维化区域和皮质小管周围毛细血管。在CsA组中,ED-1和α - sma主要在HA积聚的纤维化区域表达。结论:HA及其结合受体的上调参与了慢性csa肾损伤间质纤维化的发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信