Investigation of enhancement effects of nicotine on cholinergic neurotransmission in isolated rabbit gastric fundus: role of antioxidants

Autonomic and Autacoid Pharmacology Pub Date : 2010-06-09 DOI:10.1111/j.1474-8673.2009.00448.x

A. Anuvarbekova, G. S. O. Fincan, I. M. Vural, S. I. Ozger, Z. S. Ercan, T. Utkan, Y. Sarioglu

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引用次数: 1

Abstract

1 Nicotine, which is tobacco alkaloid, still induces interests for researchers because of smokers addiction to nicotine. Nicotine having influence on the neuronal acetylcholine receptors (nAChRs) increases release of most certain neurotransmitters from the nerve endings. Also, nicotine, affecting the mitochondrial respiratory chains, contributes to the formation of reactive oxygen species.

2 In the present study, we investigated the effects of nicotine on smooth muscles of gastric fundus on the electrical field stimulation (EFS) that induces transition contraction via stimulation nAChRs. In addition, we aimed to investigate the interaction between release of acetylcholine, induced by nicotine, and the effects of reactive oxygen species.

3 Therefore, the effects of allopurinol (10⁻⁶–10⁻⁵ m), deferoxamine (10⁻⁴ m) and mannitol (10⁻⁴–5 × 10⁻³ m) were tested on the transient contraction induced by nicotine.

4 In conclusion, mannitol (5 × 10⁻³ m) significantly reduced contractile response to nicotine on EFS only in high concentration. Whereas in small concentrations mannitol (10⁻⁴ m) statistically did not cause any results. Deferoxamine and allopurinol also did not have any significant response.

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尼古丁对离体兔胃底胆碱能神经传递增强作用的研究:抗氧化剂的作用

尼古丁是一种烟草生物碱，由于吸烟者对尼古丁上瘾，它仍然引起研究者的兴趣。尼古丁对神经元乙酰胆碱受体(nAChRs)的影响增加了神经末梢大多数神经递质的释放。此外，尼古丁影响线粒体呼吸链，有助于活性氧的形成。在本研究中，我们研究了尼古丁对胃底平滑肌的电场刺激(EFS)，通过刺激nachr诱导过渡收缩的影响。此外，我们还研究了尼古丁诱导乙酰胆碱释放与活性氧作用之间的相互作用。因此，我们测试了别嘌呤醇(10−6-10−5 m)、去铁胺(10−4 m)和甘露醇(10−4 - 5 × 10−3 m)对尼古丁引起的短暂性收缩的影响。综上所述，甘露醇(5 × 10−3 m)仅在高浓度时显著降低了EFS对尼古丁的收缩反应。而在小浓度甘露醇(10 - 4 m)中，统计上没有引起任何结果。去铁胺和别嘌呤醇也没有明显的反应。

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Autonomic and Autacoid Pharmacology

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